中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
28期
4444-4449
,共6页
胡昆鹏%刘波%姚志成%林继宗%邓美海%潘卫东%林楠%陈骋%许瑞云
鬍昆鵬%劉波%姚誌成%林繼宗%鄧美海%潘衛東%林楠%陳騁%許瑞雲
호곤붕%류파%요지성%림계종%산미해%반위동%림남%진빙%허서운
干细胞%骨髓干细胞%肝星状细胞%骨髓间充质干细胞%凋亡%肝纤维化%肝硬化%基因芯片
榦細胞%骨髓榦細胞%肝星狀細胞%骨髓間充質榦細胞%凋亡%肝纖維化%肝硬化%基因芯片
간세포%골수간세포%간성상세포%골수간충질간세포%조망%간섬유화%간경화%기인심편
bone marrow%mesenchymal stem cells%hepatic stel ate cells%apoptosis%microchip analytical procedures
背景：前期研究证实骨髓间充质干细胞可在体外促进肝星状细胞凋亡、抑制其活性，但其作用机制尚未明确。目的：通过基因芯片技术，筛选出骨髓间充质干细胞调控肝星状细胞凋亡过程中可能参与的凋亡相关基因。方法：将分离提纯的人骨髓间质干细胞与肝星状细胞在6孔 Transwel 板上建立上下共培养体系，单独培养人骨髓间质干细胞作为对照组，培养72 h。基因芯片分析单独培养组与共培养组骨髓间充质干细胞凋亡相关基因的改变情况，找出与调控肝星状细胞密切相关的凋亡基因。结果与结论：采用SABiosciences第2代功能分类基因芯片产品进行凋亡基因筛查发现，共培养后骨髓间质干细胞中显著上调的凋亡基因有：AKT1，PIK3R2，DAPK1，DHCR24，NOTCH2，BDNF等。结合前期研究结果，推测NOTCH有可能在骨髓间充质干细胞调控肝星状细胞过程中起关键作用。
揹景：前期研究證實骨髓間充質榦細胞可在體外促進肝星狀細胞凋亡、抑製其活性，但其作用機製尚未明確。目的：通過基因芯片技術，篩選齣骨髓間充質榦細胞調控肝星狀細胞凋亡過程中可能參與的凋亡相關基因。方法：將分離提純的人骨髓間質榦細胞與肝星狀細胞在6孔 Transwel 闆上建立上下共培養體繫，單獨培養人骨髓間質榦細胞作為對照組，培養72 h。基因芯片分析單獨培養組與共培養組骨髓間充質榦細胞凋亡相關基因的改變情況，找齣與調控肝星狀細胞密切相關的凋亡基因。結果與結論：採用SABiosciences第2代功能分類基因芯片產品進行凋亡基因篩查髮現，共培養後骨髓間質榦細胞中顯著上調的凋亡基因有：AKT1，PIK3R2，DAPK1，DHCR24，NOTCH2，BDNF等。結閤前期研究結果，推測NOTCH有可能在骨髓間充質榦細胞調控肝星狀細胞過程中起關鍵作用。
배경：전기연구증실골수간충질간세포가재체외촉진간성상세포조망、억제기활성，단기작용궤제상미명학。목적：통과기인심편기술，사선출골수간충질간세포조공간성상세포조망과정중가능삼여적조망상관기인。방법：장분리제순적인골수간질간세포여간성상세포재6공 Transwel 판상건립상하공배양체계，단독배양인골수간질간세포작위대조조，배양72 h。기인심편분석단독배양조여공배양조골수간충질간세포조망상관기인적개변정황，조출여조공간성상세포밀절상관적조망기인。결과여결론：채용SABiosciences제2대공능분류기인심편산품진행조망기인사사발현，공배양후골수간질간세포중현저상조적조망기인유：AKT1，PIK3R2，DAPK1，DHCR24，NOTCH2，BDNF등。결합전기연구결과，추측NOTCH유가능재골수간충질간세포조공간성상세포과정중기관건작용。
BACKGROUND:Previous studies have confirmed that bone marrow mesenchymal stem cells in vitro can promote hepatic stel ate cellapoptosis and inhibit its activity, in which the mechanism of action remains unknown. OBJECTIVE:To screen out apoptosis-related genes during hepatic stel ate cellapoptosis regulated by bone marrow mesenchymal stem cells using gene chip technology. METHODS:Purified human bone marrow mesenchymal stem cells were seeded in 6-wel Transwel plate and cocultured with hepatic stel ate cells. Cultured human bone marrow mesenchymal stem cells alone served as control group, and cultured for 72 hours. The alterations in apoptosis-related genes were analyzed between culture alone group and coculture group using gene chip technology. The genes strongly associated with regulation of hepatic stel ate cells were selected. RESULTS AND CONCLUSION:By the functional classification of second-generation SABiosciences Gene chips, apoptotic gene screening found that after coculture, significantly upregulated genes in bone marrow mesenchymal stem cells contained:AKT1, PIK3R2, DAPK1, DHCR24, NOTCH2 and BDNF. Combined with previous findings, we hypothesized that NOTCH may play a key role in the regulation of hepatic stel ate cells by bone marrow mesenchymal stem cells.
