CAJ | 학술논문

背景：研究表明，在一定条件下骨髓间充质干细胞可诱导分化为肝样细胞，为治疗急性肝衰竭等终末期肝病提供了新的思路。目的：对骨髓间充质干细胞的发现、分离培养、诱导分化及应用前景等方面做一综述。方法：计算机检索中国期刊网全文数据库以及PubMed数据库1999至2014年期间有关骨髓间充质干细胞及其向肝样细胞分化的文章。检索词分别为“肝样细胞，骨髓间充质干细胞，细胞分化”和“hepatocyte-like cel s， bone marrow mesenchymal stem cel s，differentiation”。最后选择52篇文章纳入结果分析。结果与结论：目前，肝组织工程的首要问题是寻找性状稳定具有肝特异性功能的种子细胞，成熟肝细胞获取难度较大，并具有产生免疫排斥反应、体外培养困难等缺陷，严重限制了肝移植的开展。骨髓间充质干细胞具有多向分化、自我更新快、易于扩增及培养等优点，被认为是最有前途的细胞来源。已有研究表明骨髓间充质干细胞在体内外均可分化为肝细胞，并具有肝细胞的合成和分泌功能。但如何大量扩增此类细胞的同时又能保持其良好的分化潜能、体外培养的最佳条件、诱导分化机制和临床应用的安全性等尚需进一步研究和探讨。
배경：연구표명，재일정조건하골수간충질간세포가유도분화위간양세포，위치료급성간쇠갈등종말기간병제공료신적사로。목적：대골수간충질간세포적발현、분리배양、유도분화급응용전경등방면주일종술。방법：계산궤검색중국기간망전문수거고이급PubMed수거고1999지2014년기간유관골수간충질간세포급기향간양세포분화적문장。검색사분별위“간양세포，골수간충질간세포，세포분화”화“hepatocyte-like cel s， bone marrow mesenchymal stem cel s，differentiation”。최후선택52편문장납입결과분석。결과여결론：목전，간조직공정적수요문제시심조성상은정구유간특이성공능적충자세포，성숙간세포획취난도교대，병구유산생면역배척반응、체외배양곤난등결함，엄중한제료간이식적개전。골수간충질간세포구유다향분화、자아경신쾌、역우확증급배양등우점，피인위시최유전도적세포래원。이유연구표명골수간충질간세포재체내외균가분화위간세포，병구유간세포적합성화분비공능。단여하대량확증차류세포적동시우능보지기량호적분화잠능、체외배양적최가조건、유도분화궤제화림상응용적안전성등상수진일보연구화탐토。
BACKGROUND:Studies have demonstrated that bone marrow mesenchymal stem cells can be induced to differentiate into hepatocyte-like cells under certain condition, which provides a new idea for the treatment of end-sate liver diseases, such as acute hepatic failure. OBJECTIVE:To review the discovery, isolation and culture, induction and differentiation, and application prospects of bone marrow mesenchymal stem cells. METHODS:A computer-based online research of CNKI and PubMed databases was performed to col ect articles about bone marrow mesenchymal stem cells and their differentiation into hepatocyte-like cells published between 1999 and 2014. The key words were“hepatocyte-like cells, bone marrow mesenchymal stem cells, celldifferentiation”in Chinese and English. Final y, 52 articles were included in result analysis. RESULTS AND CONCLUSION:Currently, the most important issue in liver tissue engineering is to find a kind of seed cells with stable traits and liver-specific functions. Mature hepatocytes are difficult to be harvested, and immune rejection and difficulty in in vitro culture also severely limit the development of liver transplantation. Bone marrow mesenchymal stem cells characterized as multiple differentiation, rapid self-renewal, easy to be cultured and proliferated have been considered as the most promising source of cells. Increasing studies have shown that bone marrow mesenchymal stem cells can differentiate into liver cells in vitro and in vivo, and differentiated cells have the synthesis and secretion functions same as hepatocytes. But how to proliferate such a large number of cells, to maintain the good differentiation potential of cells, to optimize the culture conditions in vitro as wel as mechanisms of induction and differentiation mechanisms and clinical security need further studies.