CAJ | 학술논문

背景：正常干细胞和肿瘤干细胞在基因表达和依赖的细胞信号通路上应该存在不同，如何发现能选择性杀伤肿瘤干细胞的治疗手段是一个仍然需要大量研究的课题。目的：分离人肝癌细胞系肿瘤干细胞MHCC97，分析其细胞生物学特性。方法：采用流式细胞技术在人高转移肝癌细胞系 MHCC97中筛选肿瘤干细胞，分离正常人肝脏干细胞CD133-CD34-MHCC97和人肝癌细胞系肿瘤干细胞CD133+CD34+MHCC97，分别检测其表型、生长曲线、细胞周期和多系分化能力。结果与结论：人肝癌细胞系 CD133+CD34+ MHCC97肿瘤干细胞的表型为 CD133+CD34+，人肝癌细胞系CD133+CD34+ MHCC97具有与CD133-CD34- MHCC97相似的细胞曲线和生长周期，可以向上皮和内皮细胞分化，并表达相应特异性的分子标志。提示人肝癌细胞系中 CD133+CD34+MHCC97细胞具有肿瘤干细胞的特性，可以向内皮和上皮细胞分化，同时具有肿瘤干细胞的生物学特性，是肿瘤复发转移的根源，也是临床治疗的靶点。
배경：정상간세포화종류간세포재기인표체화의뢰적세포신호통로상응해존재불동，여하발현능선택성살상종류간세포적치료수단시일개잉연수요대량연구적과제。목적：분리인간암세포계종류간세포MHCC97，분석기세포생물학특성。방법：채용류식세포기술재인고전이간암세포계 MHCC97중사선종류간세포，분리정상인간장간세포CD133-CD34-MHCC97화인간암세포계종류간세포CD133+CD34+MHCC97，분별검측기표형、생장곡선、세포주기화다계분화능력。결과여결론：인간암세포계 CD133+CD34+ MHCC97종류간세포적표형위 CD133+CD34+，인간암세포계CD133+CD34+ MHCC97구유여CD133-CD34- MHCC97상사적세포곡선화생장주기，가이향상피화내피세포분화，병표체상응특이성적분자표지。제시인간암세포계중 CD133+CD34+MHCC97세포구유종류간세포적특성，가이향내피화상피세포분화，동시구유종류간세포적생물학특성，시종류복발전이적근원，야시림상치료적파점。
BACKGROUND:Normal stem cells and tumor stem cells have differences in cellsignaling pathway of gene expression and dependence. How to find a therapeutic method of selectively kil ing tumor stem cells is a topic that should be studied greatly. OBJECTIVE:To isolate human liver cancer stem cells MHCC97 and to investigate the biological characteristics of them. METHODS:Tumor stem cells were selected from highly metastatic human hepatoma cellline MHCC97 by flow cytometry. CD133-CD34-MHCC97 from normal human liver stem cells and CD133+CD34+MHCC97 from tumor stem cells were isolated. Their phenotypes, growth curve, cellcycle and multi-lineage differentiation were measured. RESULTS AND CONCLUSION:Phenotypes of CD133+CD34+MHCC97 cancer stem cells were CD133+and CD34+and they had the same growth curve and cellcycle with the liver stem cells CD133-CD34-MHCC97, besides, they had the differential ability to epithelial cells and endothelial cells that proved to be stem cells. These results indicated that human cancer stem cells CD133+CD34+MHCC97 had the specific characteristics of tumor stem cells, could differentiate into epithelial cells and endothelial cells, had the biological property of tumor stem cells, was an origin of tumor relapse and metastasis, and a target of clinical therapy.