CAJ | 학술논문

目的：对我院一名患者左膝关节脓液中分离到的1株少见病原菌进行16S rRNA基因测序鉴定，探讨该方法在临床病原菌鉴定方面的意义。方法：采用16S rRNA基因序列的测定、细菌形态学以及商品化的Vitek 2全自动细菌鉴定药敏系统、API 20NE、API 20E与API 50CH系统的生化实验进行细菌的表型鉴定。结果：该菌在血平板和巧克力平板生长缓慢，麦康凯平板不生长；血平板上菌落形态呈1～2 mm“油煎蛋”圆形凸起，边缘光滑、半透明、湿润；镜下革兰染色阴性，呈链状排列，菌体1～3μm，呈圆形、卵圆形或梭形等；Vitek 2 GN-13、API 20NE、API 20E均为不能鉴定的细菌；16S rRNA基因序列分析的结果显示该菌与念珠状链杆菌的相似度为100％。结论：该膝关节脓液分离出的病原菌为念珠状链杆菌，16S rRNA基因序列分析能提供有力的遗传学证据，结合细菌的生化反应可准确地鉴定该菌。
목적：대아원일명환자좌슬관절농액중분리도적1주소견병원균진행16S rRNA기인측서감정，탐토해방법재림상병원균감정방면적의의。방법：채용16S rRNA기인서렬적측정、세균형태학이급상품화적Vitek 2전자동세균감정약민계통、API 20NE、API 20E여API 50CH계통적생화실험진행세균적표형감정。결과：해균재혈평판화교극력평판생장완만，맥강개평판불생장；혈평판상균락형태정1～2 mm“유전단”원형철기，변연광활、반투명、습윤；경하혁란염색음성，정련상배렬，균체1～3μm，정원형、란원형혹사형등；Vitek 2 GN-13、API 20NE、API 20E균위불능감정적세균；16S rRNA기인서렬분석적결과현시해균여념주상련간균적상사도위100％。결론：해슬관절농액분리출적병원균위념주상련간균，16S rRNA기인서렬분석능제공유력적유전학증거，결합세균적생화반응가준학지감정해균。
Objective To indentify Streptobacillus moniliformis isolated from the knee joint pus by 16S rRNA gene sequencing and biochemical reactions and explore the clinical value of the method. Methods The bacterial 16S rRNA gene sequence-based identification, bacterial morphology, VITEK 2 automate systems, API 20NE strips, API 20E strips and API 50CH were performed to identify the rare bacteria. Results The bacteria grew slow on blood agar and chocolate agar and were inhibited on Maconkey agar. The bacterial colony on blood agar tookes the form of 1~2 mm"omelette", which was translucent and moist with circular protrusion and smooth edges. They were Gram-staining negative and in catenation, its thalli 1~3μm, round, oval or fusiform. Vitek 2 GN-13, API 20NE and API 20E were unable to reach the identification of the bacteria. 16S rRNA gene sequencing showed the bacteria were similar to streptobacillus moniliformis by 100%. Conclusion The rare bacteria isolated from left knee joint are streptobacillus moniliformis. 16S rRNA gene sequences combined with the biochemical reactions is accurate in the identification of these bacteria.