CAJ | 학술논문

目的：利用microRNA芯片研究急性脑梗死和脑出血患者循环血浆中是否存在相关的miRNAs及有表达差异的miRNAs。方法：分别收集急性脑梗死、脑出血患者和健康对照者全血各3例，运用Norgen血浆外泌体RNA提取试剂盒提取血浆总RNA并通过定量PCR进行质量和浓度检测。采用μParaflorTM microRNA芯片技术进行miRNAs表达谱研究。用相关软件和统计学方法进行数据分析。结果：在脑梗死和脑出血患者血浆中发现8个与脑卒中相关的miRNAs，且有显著差异性表达（P＜0．05）。脑梗死和健康对照者、脑出血和健康对照者间分别有11、24个差异表达miRNAs，但两两比较上调或下调2倍以上的差异表达的miRNAs均无统计学意义（P＞0．05）。结论：脑梗死和脑出血组间miRNA的表达差异可能与不同类型脑卒中的发生发展相关，可为进一步研究急性脑卒中发生的表观遗传学分子机制提供依据。
목적：이용microRNA심편연구급성뇌경사화뇌출혈환자순배혈장중시부존재상관적miRNAs급유표체차이적miRNAs。방법：분별수집급성뇌경사、뇌출혈환자화건강대조자전혈각3례，운용Norgen혈장외비체RNA제취시제합제취혈장총RNA병통과정량PCR진행질량화농도검측。채용μParaflorTM microRNA심편기술진행miRNAs표체보연구。용상관연건화통계학방법진행수거분석。결과：재뇌경사화뇌출혈환자혈장중발현8개여뇌졸중상관적miRNAs，차유현저차이성표체（P＜0．05）。뇌경사화건강대조자、뇌출혈화건강대조자간분별유11、24개차이표체miRNAs，단량량비교상조혹하조2배이상적차이표체적miRNAs균무통계학의의（P＞0．05）。결론：뇌경사화뇌출혈조간miRNA적표체차이가능여불동류형뇌졸중적발생발전상관，가위진일보연구급성뇌졸중발생적표관유전학분자궤제제공의거。
Objective To explore the peripheral blood circulating microRNA expression profile of ischemic and hemorrhagic stroke patients by using microRNA array. Methods The whole blood of 3 patients with acute ischemic stroke, 3 patients with cerebral hemorrhage and 3 healthy volunteers were collected for whole blood RNA through Norgen exosome isolation kit, and then underwent quality and density measurement through PCR. The miRNAs expression profile was studied through μParaflorTM microRNA. All the data were analyzed by associated software and statistics methods. Results 8 miRNAs associated with cerebral stroke in ischemia stroke patients and cerebral hemorrhage patients were discovered, and showed significant difference (P<0.05). 11 differential expression miRNAs were found between ischemia stroke patients and control group, and 24 were found between hemorrhage patients and control group. However, after up-regulation, no significant difference was found (P > 0.05). Conclusions Different expression profiles of miRNA between ischemic stroke patients and hemorrhagic stroke patients may provide insights into their roles in the pathogenesis and progression of stroke. The study of miRNA can contributes to elucidate the epigenetics molecular mechanism of acute stroke.