CAJ | 학술논문

目的：评价姜黄素对糖尿病痛性周围神经病变（DPN）大鼠脊髓炎症因子的影响。方法：腹腔注射1％链脲佐菌素（ STZ ）60 mg/kg诱导大鼠DPN模型，在造模成功的大鼠中取20只随机分为糖尿病组（D 组，n＝10）和姜黄素治疗组（C组，n＝10），另取10只同月龄大鼠为空白对照组（N组，n＝10）。C、D组在第28天后分别每天给予200 mg/kg的姜黄素和等剂量生理盐水灌胃2周。于注射STZ前（T1）、注射STZ后2、7、14、21、28、35、42 d（T2～8）测定尾静脉血糖浓度；于T1、T3～8时测定大鼠右后爪缩足机械反应阈值（PWT）；于T8时处死大鼠，取腰段脊髓检测TNF-α和IL-6的含量。结果：与N组比较，D和C组T2～8时血糖浓度升高（P ＜0．05），T4～8时PWT降低（P ＜0．01）；与D组比较，C组T7、8时PWT升高（P ＜0．05）；与T1时比较，D和C组T2～8时血糖浓度升高（P ＜0．05）；与T6时比较，T7、8时C组PWT升高（P＜0．05）。与N组比较，D和C组大鼠脊髓TNF-α和IL-6含量升高（P＜0．05）；与D组比较，C组大鼠脊髓TNF-α和IL-6的含量均下降（P＜0．05）。结论：姜黄素改善DPN大鼠的疼痛，其机制可能与降低脊髓炎症因子有关。
목적：평개강황소대당뇨병통성주위신경병변（DPN）대서척수염증인자적영향。방법：복강주사1％련뇨좌균소（ STZ ）60 mg/kg유도대서DPN모형，재조모성공적대서중취20지수궤분위당뇨병조（D 조，n＝10）화강황소치료조（C조，n＝10），령취10지동월령대서위공백대조조（N조，n＝10）。C、D조재제28천후분별매천급여200 mg/kg적강황소화등제량생리염수관위2주。우주사STZ전（T1）、주사STZ후2、7、14、21、28、35、42 d（T2～8）측정미정맥혈당농도；우T1、T3～8시측정대서우후조축족궤계반응역치（PWT）；우T8시처사대서，취요단척수검측TNF-α화IL-6적함량。결과：여N조비교，D화C조T2～8시혈당농도승고（P ＜0．05），T4～8시PWT강저（P ＜0．01）；여D조비교，C조T7、8시PWT승고（P ＜0．05）；여T1시비교，D화C조T2～8시혈당농도승고（P ＜0．05）；여T6시비교，T7、8시C조PWT승고（P＜0．05）。여N조비교，D화C조대서척수TNF-α화IL-6함량승고（P＜0．05）；여D조비교，C조대서척수TNF-α화IL-6적함량균하강（P＜0．05）。결론：강황소개선DPN대서적동통，기궤제가능여강저척수염증인자유관。
Objective To evaluate the effect of curcumin on spinal inflammatory factor in rats with diabetic neuropathy. Methods Diabetic neuropathy was induced by intraperitoneal injection with 1% STZ (60 mg/kg) in sprague-dawley rats. These diabetic rats were randomly allocated to diabetic group (D group, n=10) and curcumin group ( C group , n = 10 ) . Another 10 age-matched normal rats served as controlled group ( N group , n = 10 ) . 28 days after STZ injection, the rats in C group received daily intragastric administration of curcumin (200 mg/kg) whereas those in D group received the same volume of normal saline for 2 weeks. Caudal vein blood glucose levels at T1( before STZ injection)and at T2-T8(2、7、14、21、28、35、42 days after STZ injection)from all rats were detected. Responses to the mechanical stimulus were measured with von Frey filament, and paw withdraw threshold (PWT) was recorded at T1 and at T3 to T8. At T8,the rats were killed and lumbar segments of spinal cord were removed to detect TNF-αand IL-6 content. Results Compared to N group, rats in both C and D group showed hyperglycemia at T2 to T8 (P<0.05) and lower PWT at T4~ 8 (P < 0.01). Compared to D group, C group showed higher PWT at T7,8(P<0.05). Both D and C group showed higher levels of blood sugar at T2 ~ 8 than that at T1 (P < 0.05). C group showed higher PWT at T7,8 than that at T6(P<0.05). Compared to N group,spinal TNF-αand IL-6 content increased in both D and C groups (P<0.05). Compared to D group, C group had reduction of TNF-αand IL-6 concentration (P < 0.05). Conclusion Curcumin can attenuate diabetic neuropathic pain on rats probably by reducing inflammatory factor in spinal cord.