CAJ | 학술논문

目的：探讨酪氨酸激酶Src在白色念珠菌感染中的作用及其机制。方法：通过Alarmarblue法检测不同浓度Src抑制剂PP2作用巨噬细胞不同时间点对细胞增殖的影响，通过荧光定量法检测巨噬细胞对FITC标记的白色念珠菌感染吞噬作用的影响，采用ELISA法检测PP2对巨噬细胞感染白色念珠菌产生细胞因子TNF-α和IL-10的影响。结果：0～33．3μmol/L的PP2作用细胞2 h后对细胞生长无明显影响。11．1、33．3μmol/L的PP2作用细胞24 h后细胞增殖率分别为78％、9％，48 h后增殖率分别为54％、13％。11．1μmol/L的PP2仅在作用巨噬细胞48 h后，抑制巨噬细胞对白色念珠菌的吞噬，此作用可能与抑制增殖相关，然而，PP2能显著抑制白色念珠菌对巨噬细胞的免疫应答，表现为显著抑制细胞因子TNF-α和IL-10的产生，有效浓度为11．1～33．3μmol/L（P＜0．01）。结论：酪氨酸激酶Src在巨噬细胞识别白色念珠菌感染中起着重要作用，尤其在细胞因子免疫应答中作用更加显著。
목적：탐토락안산격매Src재백색념주균감염중적작용급기궤제。방법：통과Alarmarblue법검측불동농도Src억제제PP2작용거서세포불동시간점대세포증식적영향，통과형광정량법검측거서세포대FITC표기적백색념주균감염탄서작용적영향，채용ELISA법검측PP2대거서세포감염백색념주균산생세포인자TNF-α화IL-10적영향。결과：0～33．3μmol/L적PP2작용세포2 h후대세포생장무명현영향。11．1、33．3μmol/L적PP2작용세포24 h후세포증식솔분별위78％、9％，48 h후증식솔분별위54％、13％。11．1μmol/L적PP2부재작용거서세포48 h후，억제거서세포대백색념주균적탄서，차작용가능여억제증식상관，연이，PP2능현저억제백색념주균대거서세포적면역응답，표현위현저억제세포인자TNF-α화IL-10적산생，유효농도위11．1～33．3μmol/L（P＜0．01）。결론：락안산격매Src재거서세포식별백색념주균감염중기착중요작용，우기재세포인자면역응답중작용경가현저。
Objective To investigate the role of tyrosine kinase Src in a murine C.albicans infection model. Methods Observed cell proliferation by alarmarblue assay at 2, 24 and 48 h after Src inhibitor PP2 treatment. Phagocytosis was determined by a fluorometric assay. Cytokine TNF-αand IL-10 production was detected by ELISA. Results The 0~33.3 μmol/L PP2 had no effect on cell proliferation after PP2 treatment for 2 h. When the PP2 treatment extended to 24 or 48 h, PP2 (11.1, 33.3μmol/L) showed significant inhibition on cell proliferation with 78%, 9%, and 54%,13%, respectively. At 48 h after 11.1μmol/L PP2 treatment, the internalization of C.albicans in macrophage is significantly inhibited, contributing to the inhibition of cell proliferation. However, the 11.1 and 33.3μmol/L PP2 significantly inhibited the cytokine TNF-αand IL-10 production during C.albicans infection (P<0.01). Conclusion Src kinase played an important role during C.albicans infection, especially for the cytokine TNF-αand IL-10 production.