CAJ | 학술논문

目的：观察胰高血糖素样肽-1（glucagon-like peptide-1， GLP-1）类似物利拉鲁肽对2型糖尿病大鼠胰腺十二指肠同源盒-1（pancreatic duodenal homeobox-1， PDX-1）基因表达水平的影响。方法：将60只雄性SD大鼠平均分为3组：正常对照组、糖尿病未治疗组及GLP-1组，正常对照组给予普通饲料喂养，糖尿病未治疗组与GLP-1组给予高脂饲料喂养，于第8周末以链脲佐菌素（Streptozotocin， STZ）30 mg/（kg·bw）腹腔注射高脂饲料喂养组，成模后GLP-1组给予利拉鲁肽200μg/kg皮下注射，每日2次共持续8周。其余2组予等量的生理盐水皮下注射。以实时荧光定量逆转录-聚合酶链反应（Real-Time fluorescent quantitative reverse transcription-polymerase chain reaction， Real-Time RT-PCR）法检测大鼠胰腺胰岛细胞PDX-1基因的表达。结果：与正常对照组比较，GLP-1组及未治疗组PDX-1 mRNA的表达量明显减低（P＜0．001），GLP-1治疗后2型糖尿病大鼠胰腺PDX-1 mRNA的表达量可明显增高（P＜0．001）。结论：GLP-1可上调2型糖尿病大鼠胰岛β细胞PDX-1基因的表达。
목적：관찰이고혈당소양태-1（glucagon-like peptide-1， GLP-1）유사물리랍로태대2형당뇨병대서이선십이지장동원합-1（pancreatic duodenal homeobox-1， PDX-1）기인표체수평적영향。방법：장60지웅성SD대서평균분위3조：정상대조조、당뇨병미치료조급GLP-1조，정상대조조급여보통사료위양，당뇨병미치료조여GLP-1조급여고지사료위양，우제8주말이련뇨좌균소（Streptozotocin， STZ）30 mg/（kg·bw）복강주사고지사료위양조，성모후GLP-1조급여리랍로태200μg/kg피하주사，매일2차공지속8주。기여2조여등량적생리염수피하주사。이실시형광정량역전록-취합매련반응（Real-Time fluorescent quantitative reverse transcription-polymerase chain reaction， Real-Time RT-PCR）법검측대서이선이도세포PDX-1기인적표체。결과：여정상대조조비교，GLP-1조급미치료조PDX-1 mRNA적표체량명현감저（P＜0．001），GLP-1치료후2형당뇨병대서이선PDX-1 mRNA적표체량가명현증고（P＜0．001）。결론：GLP-1가상조2형당뇨병대서이도β세포PDX-1기인적표체。
Objective To observe the effect of liraglutide, an analogue of glucagon like peptide-1 (GLP-1), on the expression of PDX-1 gene in the islet beta cells of type 2 diabetes rats. Methods Sixty male SD rats were divided into 3 groups:normal control group, diabetic model group and GLP-1 group. Normal control group was given normal diet and diabetic model group and GLP-1 group were given high fat diet. After the eighth weekend, the high fat diet group was intraperitoneally injected with streptozotocin (STZ) 30 mg/(kg·bw), and the GLP-1 group was subcutaneously injected with liraglutide 200μg/kg with 2 times per day for 8 weeks. The other groups were treated with normal saline by subcutaneous injection. Real-time fluorescent quantitative reverse transcription polymerase chain reaction (Real-Time RT-PCR) was used to detect the expression of PDX-1 gene in rat pancreatic islet cells. Results The expressions of PDX-1 mRNA in the GLP-1 group and untreated group were significantly lower (P <0.001) than that in the normal control group, but significantly higher than that after GLP-1treatment (P < 0.001). Conclusion GLP-1 can upregulate the expression of PDX-1 gene in the islet beta cells of type 2 diabetes rats.