CAJ | 학술논문

目的：比较5种体外药敏方法测定替加环素对耐亚胺培南鲍曼不动杆菌（IRAB）药敏结果的差异。方法：分别采用5种不同药敏方法检测32株IRAB对替加环素的敏感性，并以宏量肉汤稀释法为参考方法，评估其他四种方法与参考方法的一致性。结果：宏量肉汤稀释法、琼脂稀释法、Vitek-2仪器法、MTS法、纸片扩散法检测32株 IRAB 对替加环素敏感率分别为34．375％，3．125％，75％，78．125％，3．125％，中介率为65．625％、81．25％、25％、21．875％、81．25％，耐药率为0％、15．625％、0％、0％、15．625％。以宏量肉汤稀释法为参考，按FDA标准，其余4种方法均无重大误差，但基本一致率与分类一致率均低于90％。结论：替加环素对IRAB有较好的抗菌活性，但不同方法之间敏感率有所不同。
목적：비교5충체외약민방법측정체가배소대내아알배남포만불동간균（IRAB）약민결과적차이。방법：분별채용5충불동약민방법검측32주IRAB대체가배소적민감성，병이굉량육탕희석법위삼고방법，평고기타사충방법여삼고방법적일치성。결과：굉량육탕희석법、경지희석법、Vitek-2의기법、MTS법、지편확산법검측32주 IRAB 대체가배소민감솔분별위34．375％，3．125％，75％，78．125％，3．125％，중개솔위65．625％、81．25％、25％、21．875％、81．25％，내약솔위0％、15．625％、0％、0％、15．625％。이굉량육탕희석법위삼고，안FDA표준，기여4충방법균무중대오차，단기본일치솔여분류일치솔균저우90％。결론：체가배소대IRAB유교호적항균활성，단불동방법지간민감솔유소불동。
Objective To evaluate the susceptibilities of different testing methods of tigecycline against IRAB. Methods MIC and inhibitory zone diameters of 32 strains of IRAB for tigecyclinc were determined by different testing methods.Data were analyzed by comparing the results from each method to those produced by the reference macro broth dilution method. Results The rates of sensitivity intermediate resistance detected by macro broth dilution method , agardilution , MTS and Vitek-2 instrument , disk diffusion method were as follows , 34.375%, 65.625%, 0%, 3.125%, 81.25%, 15.625%, 75%, 25%, 0%, 78.125%, 21.875%, 0%, 3.125%, 81.25%, 15.625%. Compared with macro broth dilution method ,the key major error rate of other methods was 0% by FDA breakpoints , but the CA and EA rates were less than 90%. Conclusions Tigecycline has good activity against the IRAB, with significant difference in susceptible rate among the different methods.