CAJ | 학술논문

目的：观察 Th22细胞在原发性胆汁性肝硬化（PBC）患者外周血中的变化，探讨其对发病的作用。方法：流式细胞术检测 PBC 患者组与健康体检组（对照组）外周血中 Th22细胞比例；ELISA 检测血浆IL-22水平，全自动生化仪检测ALT、AST、GGT、TBIL和CRP等临床实验室指标，并进行相关分析。结果：PBC患者组Th22细胞比例明显高于对照组（P＜0．05），且肝硬化的 PBC 患者组高于非肝硬化的 PBC 患者组（P ＜0．05）；血浆 IL-22表达水平，临床指标 ALT、AST、GGT、TBIL 和 CRP， PBC 患者组明显高于对照组（P ＜0．05），且Th22细胞比例与AST，ALT、GGT 和 CRP 呈正相关（P＜0．05）。结论：PBC 患者 Th22细胞可能参与PBC的发病机制，有可能成为一个潜在的治疗靶点。
목적：관찰 Th22세포재원발성담즙성간경화（PBC）환자외주혈중적변화，탐토기대발병적작용。방법：류식세포술검측 PBC 환자조여건강체검조（대조조）외주혈중 Th22세포비례；ELISA 검측혈장IL-22수평，전자동생화의검측ALT、AST、GGT、TBIL화CRP등림상실험실지표，병진행상관분석。결과：PBC환자조Th22세포비례명현고우대조조（P＜0．05），차간경화적 PBC 환자조고우비간경화적 PBC 환자조（P ＜0．05）；혈장 IL-22표체수평，림상지표 ALT、AST、GGT、TBIL 화 CRP， PBC 환자조명현고우대조조（P ＜0．05），차Th22세포비례여AST，ALT、GGT 화 CRP 정정상관（P＜0．05）。결론：PBC 환자 Th22세포가능삼여PBC적발병궤제，유가능성위일개잠재적치료파점。
Objective To investigate the change of Th22 cells in the peripheral blood of the patients with primary biliary cirrhosis (PBC) and evaluate its clinical significance. Methods The proportion of Th22 cells in the peripheral blood of PBC patients and healthy controls were evaluated by flow cytometry. The cytokine IL-22 of each group was measured by ELISA and ALT, AST, GGT, TBIL and CRP were measured by Automatic biochemical analyzer. The proportion of Th22 cells correlation with IL-22 , ALT, AST, GGT, TBIL and CRP were analyzed. Results The proportion of Th22 cells was higher in PBC patients than healthy controls (P < 0.05), Moreover the frequency of Th22 was increased in PBC patients with liver cirrhosis than in PBC patients with liver non-cirrhosis (P < 0.05). The level of IL-22, ALT, AST, GGT, TBIL and CRP were increased in PBC patients (P < 0.05). Moreover Th22 frequency of peripheral blood was positively associated with IL-22, ALT, AST, GGT and CRP (P < 0.05). Conclusion Th22 may be involved in the pathogenesis of and it is a potential therapy target for PBC.