CAJ | 학술논문

目的：研究合肥市不同类型乙型肝炎病毒（HBV）感染者血清中 HBV 基因型和亚型分布情况及其临床意义。方法：收集192例合肥市HBV感染者血清，提取HBV 基因组DNA ，通过对PCR 扩增的HBV产物电泳及其酶切后电泳片段大小确定其基因型及其基因亚型。结果：192份血清中，82例B型，其中57例为Ba亚型，8例为Bj亚型，17例为非Ba/Bj亚型；99例为C 型，其中8例为C1亚型，56例为C2亚型，35例为非C1/C2亚型；11例为B型与C型混合感染，其中6例为Ba与C2亚型混合感染，2例为Bj与C2型混合感染，2例为Ba与非C1/C2亚型混合感染，1例为非Ba/Bj亚型与C2亚型混合感染。不同类型HBV感染者中基因型分布有差异，在肝硬化和肝细胞癌患者中C 型所占比例较高，无症状携带者中B型所占比例较高。在HBeAg 阳性和阴性感染者中，HBV基因型分布差异也有统计学意义。结论：该市HBV感染者中，以Ba和C2亚型占优势，C基因型可能与肝硬化和肝细胞癌的发生有关。
목적：연구합비시불동류형을형간염병독（HBV）감염자혈청중 HBV 기인형화아형분포정황급기림상의의。방법：수집192례합비시HBV감염자혈청，제취HBV 기인조DNA ，통과대PCR 확증적HBV산물전영급기매절후전영편단대소학정기기인형급기기인아형。결과：192빈혈청중，82례B형，기중57례위Ba아형，8례위Bj아형，17례위비Ba/Bj아형；99례위C 형，기중8례위C1아형，56례위C2아형，35례위비C1/C2아형；11례위B형여C형혼합감염，기중6례위Ba여C2아형혼합감염，2례위Bj여C2형혼합감염，2례위Ba여비C1/C2아형혼합감염，1례위비Ba/Bj아형여C2아형혼합감염。불동류형HBV감염자중기인형분포유차이，재간경화화간세포암환자중C 형소점비례교고，무증상휴대자중B형소점비례교고。재HBeAg 양성화음성감염자중，HBV기인형분포차이야유통계학의의。결론：해시HBV감염자중，이Ba화C2아형점우세，C기인형가능여간경화화간세포암적발생유관。
Objective To study the distribution and its clinical significance of hepatitis B virus genotypes and subtypes in HBV-infected patients in Hefei. Methods 192 serum samples from HBV-infected patients were collected to extract HBV DNA. HBV the genotypes and subtypes of HBV were detected by PCR. Results Among 192 HBV-infected patients, 82 had genotype B, 99 had genotype C, and 11 had B/C. In genotype B, 57 were subtype Ba, 8 were subtype Bj, and the remaining 17 were neither Ba nor Bj. In genotype C, 8 were subtype C1, 56 were subtype C2, and the other 35 were neither C1 nor C2. In genotype B/C, 6 were Ba/C2, 2 Bj/C2, 2 Ba/neither C1 nor C2, and one neither Ba nor Bj/C2. Tthe distribution of HBV genotypes and subtypes exhibited significant differences in the various clinical types; and it showed that genotype C was predominant among patients with liver cirrhosis and hepatocellular carcinoma , while genotype B was predominant in asymptomatic carriers. In addition, there were no differences in HBV genotype distribution among patient wih different gender; but the genotype distribution differed significantly between patients positive with HBeAg and those negative with HBeAg. Conclusions Subtypes Ba and C2 are predominant in HBV-infected patients in Hefei. Genotype C may be associated with the development of liver cirrhosis and hepatocellular carcinoma.