CAJ | 학술논문

目的：研究在上皮性卵巢癌组织中母系表达基因（MEG3）基因启动子异常甲基化状态与其临床病理特征的关系。方法：运用甲基化特异性聚合酶链反应（MSP）法，检测47例卵巢癌组织（蜡块标本）及15例正常卵巢组织（蜡块标本）中MEG3基因启动子甲基化状态，分析其甲基化状态与临床病理特征的关系。结果：MEG3基因甲基化发生率在卵巢癌组织（42．6％）中高于正常卵巢组织（13．3％），差异有统计学意义（P＝0．035）；年龄＞60岁患者MEG3基因甲基化发生率略高于年龄≤60岁，差异无统计学意义（P＞0．05）；临床Ⅲ～Ⅳ期患者MEG3基因甲基化的发生率低于临床Ⅰ～Ⅱ期，差异无统计学意义（P＞0．05）；MEG3基因甲基化阳性率在卵巢癌不同病理分级和组织学类型间未见明显差异（P＞0．05）。结论：MEG3基因异常甲基化可能与上皮性卵巢癌发生相关，与其临床病理无关。
목적：연구재상피성란소암조직중모계표체기인（MEG3）기인계동자이상갑기화상태여기림상병리특정적관계。방법：운용갑기화특이성취합매련반응（MSP）법，검측47례란소암조직（사괴표본）급15례정상란소조직（사괴표본）중MEG3기인계동자갑기화상태，분석기갑기화상태여림상병리특정적관계。결과：MEG3기인갑기화발생솔재란소암조직（42．6％）중고우정상란소조직（13．3％），차이유통계학의의（P＝0．035）；년령＞60세환자MEG3기인갑기화발생솔략고우년령≤60세，차이무통계학의의（P＞0．05）；림상Ⅲ～Ⅳ기환자MEG3기인갑기화적발생솔저우림상Ⅰ～Ⅱ기，차이무통계학의의（P＞0．05）；MEG3기인갑기화양성솔재란소암불동병리분급화조직학류형간미견명현차이（P＞0．05）。결론：MEG3기인이상갑기화가능여상피성란소암발생상관，여기림상병리무관。
Objective To study the relationship between the methylation status of CPG islands in MEG3 gene promoter region of epithelial ovarian cancer and its clinical and pathological features. Methods The promoter methylation status was evaluated by MSP (methylation-specific polymerase chain reaction ) in 47 cases of ovarian cancer tissue and 15 cases of normal control. Results The methylation ratio (42.6%) of the MEG3 genes in the ovarian cancer was statistically significantly higher (P = 0.035 ) than that (13.3%) in the normal control. The methyation rate of the group with an age > 60 years old was slightly higher than that of the group with an age≤60 years old, without statistically significant (P > 0.05), so was observed in ovarian cancers of stage Ⅰ andⅡ than that in stage Ⅲ and Ⅳ. There were also no significant differences in MEG3 gene methylation positive rate neither in different pathological grading nor in various ovarian cancer tissues (P > 0.05). Conclusion Abnormal methylation in MEG3 gene may be associated with epithelial ovarian cancer , but no relation to its clinical pathology.