CAJ | 학술논문

目的：探讨hsa-miR-181a（miR-181a）在子宫内膜癌变过程中的表达及其意义。方法：选取甲醛固定石蜡包埋组织75例，其中正常子宫内膜13例，子宫内膜增生症18例，子宫内膜癌44例。提取总RNA ，采用实时荧光定量PCR技术检测各组子宫内膜组织中miR-181a 的表达量。结果：在甲醛固定石蜡包埋组织中可检测到miR-181a的表达。 miR-181a在子宫内膜癌组织中的表达高于子宫内膜增生症，在子宫内膜增生症组织中的表达高于正常子宫内膜，差异均有统计学意义（P＜0．05）；miR-181a 的表达与FIGO分期有关，差异有统计学意义（P＜0．05）。结论：miR-181a 在子宫内膜癌组织中表达上调，可能起到了癌基因的作用；miR-181a在子宫内膜癌变过程中的差异表达可能与子宫内膜癌的发生、发展有关。
목적：탐토hsa-miR-181a（miR-181a）재자궁내막암변과정중적표체급기의의。방법：선취갑철고정석사포매조직75례，기중정상자궁내막13례，자궁내막증생증18례，자궁내막암44례。제취총RNA ，채용실시형광정량PCR기술검측각조자궁내막조직중miR-181a 적표체량。결과：재갑철고정석사포매조직중가검측도miR-181a적표체。 miR-181a재자궁내막암조직중적표체고우자궁내막증생증，재자궁내막증생증조직중적표체고우정상자궁내막，차이균유통계학의의（P＜0．05）；miR-181a 적표체여FIGO분기유관，차이유통계학의의（P＜0．05）。결론：miR-181a 재자궁내막암조직중표체상조，가능기도료암기인적작용；miR-181a재자궁내막암변과정중적차이표체가능여자궁내막암적발생、발전유관。
Objective To explore the expression and significance of hsa-miR-181a (miR-181a) in can-ceration progression of endometrial carcinoma. Methods A total of 75 formalin-fixed paraffin-embedded tissue specimens were studied in this study , of which , 13 were normal endometrium , 18 were endometrial hyperplasia , 44 were endometrial carcinoma. After total RNA had been extracted , real-time PCR was applied to detect the ex-pression level of miR-181a in endometrial tissue in each group. Results miR-181a expression in formalin-fixed paraffin-embedded tissue specimens can be detected. Expression of miR-181a in endometrial carcinoma was high-er than that in endometrial hyperplasia , its expression in endometrial hyperplasia was also higher than that in normal endometrium, and the difference was statistically significant (P < 0.05). The expression of miR-181a in endometrial carcinoma was associated with FIGO stages (P < 0.05). Conclusion The up-regulation of miR-181a expression in women with endometrial carcinoma may play the role of oncogenes. Abnormal expression of miR-181a is probably associated with the occurrence and development of endometrial carcinoma.