CAJ | 학술논문

目的：检测慢性阻塞性肺疾病（COPD）大鼠肺组织中 apelin 及其受体 APJ 的 mRNA 和蛋白表达，探讨apelin-APJ系统在COPD发生、发展中的作用。方法：烟雾暴露法制造COPD大鼠模型。实验设对照组和COPD组。 COPD组大鼠每日2次在熏烟箱内烟雾暴露，每次连续被动吸烟20支（1 h），持续4个月。qRT-PCR测定大鼠肺组织中apelin mRNA及APJ mRNA表达。免疫组化法检测大鼠肺组织apelin和APJ蛋白表达。结果：COPD组apelin、APJ mRNA相对表达量显著低于对照组（P ＜0．01）。大鼠肺组织apelin、APJ mRNA 表达与 RV/（LV ＋ S）均呈负相关（r ＝-0．454、-0．448，均 P ＜0．05）；与 FEV0．3/FVC 呈正相关（r ＝0．529、0．475，均P ＜0．05）。 apelin、APJ蛋白主要表达在支气管肺组织上皮细胞、肺泡巨噬细胞、血管内皮细胞及部分肺泡壁细胞的细胞膜和细胞浆，COPD组及对照组apelin、APJ蛋白均呈阳性表达，半定量检测COPD组表达低于对照组（P＜0．05）。结论：apelin及其受体APJ的 mRNA和蛋白在COPD大鼠肺组织表达均下降，可能是导致大鼠COPD肺动脉高压、肺心病发生发展的重要因素。 apelin-APJ系统可能成为探索防治COPD肺动脉高压、右心室肥大的新靶点。
목적：검측만성조새성폐질병（COPD）대서폐조직중 apelin 급기수체 APJ 적 mRNA 화단백표체，탐토apelin-APJ계통재COPD발생、발전중적작용。방법：연무폭로법제조COPD대서모형。실험설대조조화COPD조。 COPD조대서매일2차재훈연상내연무폭로，매차련속피동흡연20지（1 h），지속4개월。qRT-PCR측정대서폐조직중apelin mRNA급APJ mRNA표체。면역조화법검측대서폐조직apelin화APJ단백표체。결과：COPD조apelin、APJ mRNA상대표체량현저저우대조조（P ＜0．01）。대서폐조직apelin、APJ mRNA 표체여 RV/（LV ＋ S）균정부상관（r ＝-0．454、-0．448，균 P ＜0．05）；여 FEV0．3/FVC 정정상관（r ＝0．529、0．475，균P ＜0．05）。 apelin、APJ단백주요표체재지기관폐조직상피세포、폐포거서세포、혈관내피세포급부분폐포벽세포적세포막화세포장，COPD조급대조조apelin、APJ단백균정양성표체，반정량검측COPD조표체저우대조조（P＜0．05）。결론：apelin급기수체APJ적 mRNA화단백재COPD대서폐조직표체균하강，가능시도치대서COPD폐동맥고압、폐심병발생발전적중요인소。 apelin-APJ계통가능성위탐색방치COPD폐동맥고압、우심실비대적신파점。
Objective To detect the expression of apelin and angiotensin I receptor related protein (APJ) in rats with chronic obstructive pulmonary disease (COPD), and to explore the role of apelin-APJ system in the occurrence and development of COPD. Methods A rat model of COPD was established using the method of smoke exposure. SD rats were randomly divided into a control group and a COPD group. The rats in COPD group were exposed to secondhand cigarette smoke in a fume box twice a day for 4 months. Expressions of apelin mRNA and APJ mRNA in rat lung tissues were detected by real-time quantitative polymerase chain reaction (qRT-PCR). Expressions of apelin protein and APJ protein were determined by immunohistochemistry. Results The expressions of apelin mRNA and APJ mRNA in COPD group were decreased by 44% and 13% as compared with those in the control group. The mRNA expressions of apelin and APJ in rat lung tissues were negatively correlated with RV/(LV + S) (r = -0.454 and -0.448, P < 0.05), and positively related with FEV0.3/FVC (r = 0.529 and 0.475;P < 0.05). Apelin and APJ were mainly expressed in the epithelial cells of the bronch and lung, alveolar macrophages, vascular endothelial cells, and the membrane and cytoplasm of some alveolar wall cells. The expression of apelin and APJ protein was lower in COPD group than in the control group (P < 0.05). Conclusions The mRNA and protein expressions of apelin and APJ are decreased in the lung tissues in rats with COPD , which may be an important factor leading to the development of COPD. Apelin-APJ system might be a new target for prevention and treatment of pulmonary hypertension and right ventricular hypertrophy induced by COPD.