实用医学杂志
實用醫學雜誌
실용의학잡지
THE JOURNAL OF PRACTICAL MEDICINE
2014年
12期
1867-1870
,共4页
岳春燕%毛欣茹%郑磊%高雅%朱阳敏%吴彬%洪佳琼%平宝红
嶽春燕%毛訢茹%鄭磊%高雅%硃暘敏%吳彬%洪佳瓊%平寶紅
악춘연%모흔여%정뢰%고아%주양민%오빈%홍가경%평보홍
模拟微重力%巨核细胞%c-mpl%GATA-1%NF-E2%RUNX-1
模擬微重力%巨覈細胞%c-mpl%GATA-1%NF-E2%RUNX-1
모의미중력%거핵세포%c-mpl%GATA-1%NF-E2%RUNX-1
Simulated microgravity%Megakaryocyte%c-mpl%GATA-1%NF-E2%RUNX1
目的：探讨模拟微重力对巨核细胞增殖和分化的影响及机制。方法：利用RCCS-4模拟微重力，采用台盼蓝染色判断细胞活力，细胞计数及 CCK8法评估细胞增殖情况，采用流式细胞术检测 CD41＋/CD61＋细胞比例和细胞周期。 RT-PCR 法检测血小板生成素受体（c-mpl）和相关转录因子mRNA 表达水平。结果：培养24、48、72 h 后，SMG组细胞计数、CCK8法细胞增殖活性、G2/M期细胞比例、c-mpl mRNA 表达水平均显著低于 NG 组（P ＜0．05）；培养48、72 h 后 SMG 组 CD41＋/CD61＋细胞比例、Rant 相关转录因子1（RUNX-1） mRNA表达水平显著低于NG组（P＜0．05）。结论：模拟微重力抑制体外培养巨核细胞增殖和分化，转录受抑导致c-mpl 表达下调进而c-mpl介导的下游通路受抑可能是相关机制。
目的：探討模擬微重力對巨覈細胞增殖和分化的影響及機製。方法：利用RCCS-4模擬微重力，採用檯盼藍染色判斷細胞活力，細胞計數及 CCK8法評估細胞增殖情況，採用流式細胞術檢測 CD41＋/CD61＋細胞比例和細胞週期。 RT-PCR 法檢測血小闆生成素受體（c-mpl）和相關轉錄因子mRNA 錶達水平。結果：培養24、48、72 h 後，SMG組細胞計數、CCK8法細胞增殖活性、G2/M期細胞比例、c-mpl mRNA 錶達水平均顯著低于 NG 組（P ＜0．05）；培養48、72 h 後 SMG 組 CD41＋/CD61＋細胞比例、Rant 相關轉錄因子1（RUNX-1） mRNA錶達水平顯著低于NG組（P＜0．05）。結論：模擬微重力抑製體外培養巨覈細胞增殖和分化，轉錄受抑導緻c-mpl 錶達下調進而c-mpl介導的下遊通路受抑可能是相關機製。
목적：탐토모의미중력대거핵세포증식화분화적영향급궤제。방법：이용RCCS-4모의미중력，채용태반람염색판단세포활력，세포계수급 CCK8법평고세포증식정황，채용류식세포술검측 CD41＋/CD61＋세포비례화세포주기。 RT-PCR 법검측혈소판생성소수체（c-mpl）화상관전록인자mRNA 표체수평。결과：배양24、48、72 h 후，SMG조세포계수、CCK8법세포증식활성、G2/M기세포비례、c-mpl mRNA 표체수평균현저저우 NG 조（P ＜0．05）；배양48、72 h 후 SMG 조 CD41＋/CD61＋세포비례、Rant 상관전록인자1（RUNX-1） mRNA표체수평현저저우NG조（P＜0．05）。결론：모의미중력억제체외배양거핵세포증식화분화，전록수억도치c-mpl 표체하조진이c-mpl개도적하유통로수억가능시상관궤제。
Objective To investigate the effect of simulated microgravity on the proliferation and differentiation of the human megakaryocyte cells in vitro. Methods The fourth generation rotating cell culture system (RCCS-4) was used to generate the simulated microgravity environment. The cell viability was assessed by trypan blue staining method. The proliferation of cells was assessed by cell counting method and CCK8 method. The CD41+/CD61+ cells rate and the cells cycle were detected by flow cytometry. The expression levels of thrombopoietin receptor (c-mpl) and transcription factors were detected with RT-PCR. Results After 24, 48, 72 h, culture under simulated microgravity resulted in a significant decrease in the cell number , proliferative activity, cells in the G2/M phase and levels of c-mpl mRNA expression in comparison with that under the normal gravity (P < 0.05). After 48 h and 72 h culture, CD41+/CD61+ cells ratio decreased and RUNX-1 mRNA expression was down-regulated in cells of the group SMG compared with that of the group NG (P < 0.05). Conclusion Microgravity can inhibit the proliferation and differentiation of human megakaryocyte cells in vitro. The mechanism may be that TPO/c-mpl pathway was inhibited by down regulating the expression of c-mpl which transcriptional inhibition lead to.
