CAJ | 학술논문

目的：研究银杏叶提取物对大鼠机械通气所致肺损伤超氧化物歧化酶（ SOD ）、丙二醛（ MDA ）的影响及机制。方法：将30只雄性SD大鼠随机分为3组：对照组（C组）、大潮气量组（H组）和银杏叶提取物组（ E 组）。H 组和 E 组设置机械通气潮气量30 mL/kg ，呼吸频率40次/min ， I/E 为1/3，呼气末正压为0 cmH2O，吸入氧浓度为21％。机械通气4 h 后收集支气管肺泡灌洗液和血清测定SOD和MDA 的水平，取肺组织测定肺湿/干重比（W/D），观察肺组织病理改变。结果：H 组与C组相比，MDA、W/D水平均显著增高，SOD水平则显著降低；E组与H组相比，大鼠MDA、W/D水平均显著降低，SOD水平则显著升高。肺组织病理改变显示E组的病理损害明显比H组减轻。结论：银杏叶提取物能减轻肺水肿，减少机械通气所致的肺损伤。
목적：연구은행협제취물대대서궤계통기소치폐손상초양화물기화매（ SOD ）、병이철（ MDA ）적영향급궤제。방법：장30지웅성SD대서수궤분위3조：대조조（C조）、대조기량조（H조）화은행협제취물조（ E 조）。H 조화 E 조설치궤계통기조기량30 mL/kg ，호흡빈솔40차/min ， I/E 위1/3，호기말정압위0 cmH2O，흡입양농도위21％。궤계통기4 h 후수집지기관폐포관세액화혈청측정SOD화MDA 적수평，취폐조직측정폐습/간중비（W/D），관찰폐조직병리개변。결과：H 조여C조상비，MDA、W/D수평균현저증고，SOD수평칙현저강저；E조여H조상비，대서MDA、W/D수평균현저강저，SOD수평칙현저승고。폐조직병리개변현시E조적병리손해명현비H조감경。결론：은행협제취물능감경폐수종，감소궤계통기소치적폐손상。
Objective To investigate the effect of EGB on SOD, MDA of ventilator-induced lung injury in rats and its possible mechanisms. Methods Thirty male SD rats were randomly divided into 3 groups: control group (C group), high tidal ventilation group (H group) and EGB group (E group). The setting mechanical ventilation was VT=30 mL/kg, RR=40/min, I/E=1/3, PEEP=0 cmH2 O and FiO2=21%. The broncho-alveolar lavage fluid (BLAF) and serum were obtained for determination of the levels of SOD and MDA at the end of 4 h mechanical ventilation. The Lungs were removed, and the wet-to-dry weight ratio (W/D) and pulmonary pathologic changes were measured. Results As compared with C group, W/D and the levels of MDA were significantly increased in H group, but the levels of SOD were reduced in H group. As compared with H group, W/D and the levels of MDA were significantly decreased in E group, but the levels of SOD were increased in E group. Pulmonary pathologic changes were alleviated in E group comparing with H group. Conclusion EGB injection may have a protective role against hyperoxia and induced pulmonary damage in rats.