CAJ | 학술논문

目的：获得大肠癌colo205细胞株不同细胞群差异基因表达信息，为后续研究提供遗传学依据。方法：提取colo205细胞及其来源的CD133＋、CD133-总RNA，进行表达谱测序，利用生物信息学软件分析测序结果。结果：得到124个差异表达基因，共涉及32个代谢途径。结论：Colo205细胞来源不同类群细胞间存在大量差异基因，可为大肠癌研究提供表观遗传学依据。
목적：획득대장암colo205세포주불동세포군차이기인표체신식，위후속연구제공유전학의거。방법：제취colo205세포급기래원적CD133＋、CD133-총RNA，진행표체보측서，이용생물신식학연건분석측서결과。결과：득도124개차이표체기인，공섭급32개대사도경。결론：Colo205세포래원불동류군세포간존재대량차이기인，가위대장암연구제공표관유전학의거。
Objective To obtain differential expression genes from colorectal cancer cells derived from colo205 for further research. Methods RNA from colo205 cells,CD133+cells and CD133-cells were sequenced and analyzed by bioinformatics software. Results One hundred and twenty four differential expression genes were obtained, which involves 32 metabolic pathways. Conclusions Large quantities of differential genes can be found among different groups of cells derived from colo205 cells , which can provide epigenetic evidence for colorectal cancer research.