CAJ | 학술논문

目的：探讨快速老化小鼠听觉功能以及耳蜗组织中神经生长因子受体酪氨酸激酶受体 A（nerve growth factor receptor TrkA，NGFR TrkA）表达的增龄性变化。方法：选用3、5、7月龄的快速老化小鼠亚系8（SAMP8）作为观察对象。应用听觉诱发反应仪检测其8 kHz短纯音听觉脑干反应阈值；应用NGFR TrkA免疫组化染色检测其耳蜗组织染色光密度进行分析。结果：SAMP8听性脑干反应阈值随着月龄增加而显著增高（P＜0．05）；NGFR TrkA蛋白在不同月龄快速老化小鼠耳蜗组织中均有表达，SAMP8耳蜗组织中的NGFR TrkA蛋白染色光密度随着月龄增加而显著降低（P＜0．05）。结论：NGFR TrkA蛋白的表达水平随着快速老化小鼠听觉功能增龄性减退而降低，说明NGFR TrkA可能与维持耳蜗的功能状态有关。
목적：탐토쾌속노화소서은각공능이급이와조직중신경생장인자수체락안산격매수체 A（nerve growth factor receptor TrkA，NGFR TrkA）표체적증령성변화。방법：선용3、5、7월령적쾌속노화소서아계8（SAMP8）작위관찰대상。응용은각유발반응의검측기8 kHz단순음은각뇌간반응역치；응용NGFR TrkA면역조화염색검측기이와조직염색광밀도진행분석。결과：SAMP8은성뇌간반응역치수착월령증가이현저증고（P＜0．05）；NGFR TrkA단백재불동월령쾌속노화소서이와조직중균유표체，SAMP8이와조직중적NGFR TrkA단백염색광밀도수착월령증가이현저강저（P＜0．05）。결론：NGFR TrkA단백적표체수평수착쾌속노화소서은각공능증령성감퇴이강저，설명NGFR TrkA가능여유지이와적공능상태유관。
Objective To explore hearing loss , the expression of age-related changes of nerve growth factor receptor TrkA (NGFR TrkA ) in the cochlea of the senescence-accelerated mouse. Methods The senescence accelerated mouse/prone 8(SAMP8) at 3, 5, 7 months were chosen as analyzing subjects. The auditory thresholds was monitored by auditory brainstem respons (ABR). The expression of NGFR TrkA protein was analyzed by the optical density of immunohistochemical staining. Results The SAMP8 developed a progressive auditory threshold which showed an age-related significant increase (P < 0.05). There were NGFR TrkA protein expressed in the cochlea of the SAMP8 throughout the development, which developed an age-related significant descend (P<0.05). Conclusion The expression level of NGFR TrkA protein decreases when the SAMP 8 develops a progressive hearing loss which indicates that NGFR TrkA protein probably has relationship with maintaining functional status of the cochlea.