CAJ | 학술논문

目的：探讨miR-19对肺腺癌A549细胞株迁移能力的作用。方法：运用qRT-PCR检测miR-19在肺上皮细胞株BEAS-2B 和肺腺癌A549-Luc 细胞株中的表达；建立稳定过表达 miR-19的 A549-Luc 肺癌细胞株；qRT-PCR鉴定稳定细胞株A549/RFP＋/ H2B 和A549/RFP＋/m19中miR-19的表达水平；观察其形态变化及Transwell小室细胞迁移实验检测细胞迁移能力变化。结果：miR-19在BEAS-2B及A549-Luc细胞中的表达差异具有显著性（t＝-20．954，P＜0．001）。成功建立了稳定过表达 miR-19的肺腺癌细胞株 A549/RFP＋/m19；观察发现A549/RFP＋/m19细胞形态发生变化，且与空载A549/RFP＋/ H2B 细胞相比，A549/RFP＋/m19细胞迁移能力增强（P＜0．001）。结论：miR-19可使肺腺癌A549-Luc细胞迁移能力增强。
목적：탐토miR-19대폐선암A549세포주천이능력적작용。방법：운용qRT-PCR검측miR-19재폐상피세포주BEAS-2B 화폐선암A549-Luc 세포주중적표체；건립은정과표체 miR-19적 A549-Luc 폐암세포주；qRT-PCR감정은정세포주A549/RFP＋/ H2B 화A549/RFP＋/m19중miR-19적표체수평；관찰기형태변화급Transwell소실세포천이실험검측세포천이능력변화。결과：miR-19재BEAS-2B급A549-Luc세포중적표체차이구유현저성（t＝-20．954，P＜0．001）。성공건립료은정과표체 miR-19적폐선암세포주 A549/RFP＋/m19；관찰발현A549/RFP＋/m19세포형태발생변화，차여공재A549/RFP＋/ H2B 세포상비，A549/RFP＋/m19세포천이능력증강（P＜0．001）。결론：miR-19가사폐선암A549-Luc세포천이능력증강。
Objective To investigate the effects of miR-19 on the migration ability of lung adenocarcinoma cell line A549. Methods The expressions of miR-19 in lung epithelial cell line BEAS-2B and lung adenocarcinoma cell line A549-Luc were detected by qRT-PCR.The A549-Luc cell line which over-expressed miR-19 was established. The expression levels of miR-19 in A549/RFP+/H2B and A549/RFP+/m19 were identified by qRT-PCR. The morphology of A549/RFP+/m19 was observed,and the migration ability of A549/RFP+/m19 was detected by transwell migration assay. Results The expression levels of miR-19 differed significantly between BEAS-2B cells and A549-Luc cells (t = -20.954, P < 0.001). The lung adenocarcinoma cell line A549/RFP+/m19 which over-expressed miR-19 was successfully established. Changes in A549/RFP+/m19 cell morphology were found. As compared with A549/RFP+/H2B cells, A549/RFP+/m19 had an increased migration ability (P <0.01). Conclusions miR-19 enhances the migration ability of lung adenocarcinoma A549-Luc cells.