食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2014年
13期
119-122
,共4页
UPLC%纳他霉素%检测%假阳性
UPLC%納他黴素%檢測%假暘性
UPLC%납타매소%검측%가양성
UPLC%natamycin%determination%false positive
建立了乳制品中纳他霉素残留量的超高效液相色谱（UPLC）检测方法，并讨论了几种常用的“假阳性”验证方法。用甲醇提取样片中的纳他霉素，采用BEH Shield RP18分离，检测波长为303 nm，以水：甲醇=55∶45为流动相进行等度洗脱，该方法前处理简单，回收率高及灵敏度高，准确度和稳定性好。
建立瞭乳製品中納他黴素殘留量的超高效液相色譜（UPLC）檢測方法，併討論瞭幾種常用的“假暘性”驗證方法。用甲醇提取樣片中的納他黴素，採用BEH Shield RP18分離，檢測波長為303 nm，以水：甲醇=55∶45為流動相進行等度洗脫，該方法前處理簡單，迴收率高及靈敏度高，準確度和穩定性好。
건립료유제품중납타매소잔류량적초고효액상색보（UPLC）검측방법，병토론료궤충상용적“가양성”험증방법。용갑순제취양편중적납타매소，채용BEH Shield RP18분리，검측파장위303 nm，이수：갑순=55∶45위류동상진행등도세탈，해방법전처리간단，회수솔고급령민도고，준학도화은정성호。
A method for the determination of natamycin in various dairy was established by ultra performance liquid chromatography (UPLC), and several false-positive confirmed methods for the identification were discussed. The sample was extracted with methanol as extraction solvents. The separation was performed on a BEH Shield RP18 column by eluent of water and methanol with their ratio 55∶45 as mobile phase. The method was simple, high recovery, sensitive, accurate and stable.
