CAJ | 학술논문

建立水产品中甲氧苄啶药物残留量测定的高效液相色谱-串联质谱方法。样品选择乙腈提取，用正己烷脱脂，固相萃取柱净化，采用LC-MS/MS选择反应监测（SRM）正离子模式测定进行定性、定量分析。结果表明：甲氧苄啶药物的检出限（LOD）为0.5μg/kg，定量限（LOQ）为1.0μg/kg，检测结果的相对标准偏差为1.78%～5.08%，加标回收率达到77.1%～93.5%。该方法具有比较高的重现性和选择性，在水产品中甲氧苄啶的残留测定中具有很好的应用前景。
건립수산품중갑양변정약물잔류량측정적고효액상색보-천련질보방법。양품선택을정제취，용정기완탈지，고상췌취주정화，채용LC-MS/MS선택반응감측（SRM）정리자모식측정진행정성、정량분석。결과표명：갑양변정약물적검출한（LOD）위0.5μg/kg，정량한（LOQ）위1.0μg/kg，검측결과적상대표준편차위1.78%～5.08%，가표회수솔체도77.1%～93.5%。해방법구유비교고적중현성화선택성，재수산품중갑양변정적잔류측정중구유흔호적응용전경。
Established an analytical method for the determination of trimethoprim residues in aquatic products by high performance liquid chromatography-tandem mass spectrometry(LC-MS/MS). The sample was extracted with acetonitrile,defatted-up by hexane, and cleaned up using mixed-mode cation exchange solid-phase extraction cartridges. The mass spectrometer was operated in the positive ion mode using selected reaction monitoring for the qualitative and quantitative analysis. The detection limits of this method for tetracyclines and clenbuterol residues were 0.5μg/kg, and the limits of quantification were 1.0μg/kg. The relative standard deviations ranging from 1.78%-5.08%and the recovery rates were from 77.1%-93.5%. The method proposed by this paper possesses higher prodicibility and selectivity, It is promising in real applications of determining the trimethoprim residues in aquatic products.