食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2014年
13期
106-109
,共4页
李琳琳%曹新志%游见明%赵迎庆
李琳琳%曹新誌%遊見明%趙迎慶
리림림%조신지%유견명%조영경
茚三酮显色反应%凯氏定氮%单宁酸%多肽得率
茚三酮顯色反應%凱氏定氮%單寧痠%多肽得率
인삼동현색반응%개씨정담%단저산%다태득솔
ninhydrin color reaction%Kjeluhl%tannic acid%yield of peptide
用茚三酮显色法测定蚕丝蛋白质水解液中游离氨基酸的含量，15%单宁酸沉淀蛋白质水解液中的大分子蛋白质，经离心过滤后，用凯氏定氮法测定上清液中总蛋白含量，继而测定蚕丝蛋白水解液中多肽的得率。本试验以期用低成本高效率的方法测得多肽的得率，进而可以应用于工业化生产。
用茚三酮顯色法測定蠶絲蛋白質水解液中遊離氨基痠的含量，15%單寧痠沉澱蛋白質水解液中的大分子蛋白質，經離心過濾後，用凱氏定氮法測定上清液中總蛋白含量，繼而測定蠶絲蛋白水解液中多肽的得率。本試驗以期用低成本高效率的方法測得多肽的得率，進而可以應用于工業化生產。
용인삼동현색법측정잠사단백질수해액중유리안기산적함량，15%단저산침정단백질수해액중적대분자단백질，경리심과려후，용개씨정담법측정상청액중총단백함량，계이측정잠사단백수해액중다태적득솔。본시험이기용저성본고효솔적방법측득다태적득솔，진이가이응용우공업화생산。
The content of free amino acid in silk protein hydrolysate is determined by using ninhydrin color reaction. Macromolecule protein in protein hydrolysate is deposited by using 15 %tannic acid. After centrifugalization and filtration, the total content of protein in the clear solution is determined by using Kjeluhl method. Then the yield of peptide in silk protein hydrolysate is obtained. The purpose of this test is to obtain the yield of peptide by using cost-efficient method , then this approach can be applied to industrial production.
