食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2014年
13期
21-23
,共3页
香椿%组织培养%工艺技术
香椿%組織培養%工藝技術
향춘%조직배양%공예기술
tissue culture%cedrela sinensis%technology
以野生香椿种子为外植体进行培养,将香椿种子用75%酒精溶液消毒20 s,再用0.1%升汞溶液消毒5 min,无菌水冲洗4次~6次,接种到培养基上。在接种8 d~10 d后外植体分化出带有一片小叶的新生枝。切取子叶和下胚轴作为外植体接种B5+蔗糖30 g/L的培养基中,10 d后形成增殖苗。通过组织培养技术,缩短香椿的生长周期,再将香椿的嫩芽做如下处理浸泡(料液比1∶4,0.05%碳酸钠)、清洗、浸烫(95℃水浴2 min,500 mg/kg的葡萄糖酸锌溶液)、沥干冷却、切碎、拌料、加入添加剂、装罐、杀菌(85℃,30 min)、冷却、贮藏。
以野生香椿種子為外植體進行培養,將香椿種子用75%酒精溶液消毒20 s,再用0.1%升汞溶液消毒5 min,無菌水遲洗4次~6次,接種到培養基上。在接種8 d~10 d後外植體分化齣帶有一片小葉的新生枝。切取子葉和下胚軸作為外植體接種B5+蔗糖30 g/L的培養基中,10 d後形成增殖苗。通過組織培養技術,縮短香椿的生長週期,再將香椿的嫩芽做如下處理浸泡(料液比1∶4,0.05%碳痠鈉)、清洗、浸燙(95℃水浴2 min,500 mg/kg的葡萄糖痠鋅溶液)、瀝榦冷卻、切碎、拌料、加入添加劑、裝罐、殺菌(85℃,30 min)、冷卻、貯藏。
이야생향춘충자위외식체진행배양,장향춘충자용75%주정용액소독20 s,재용0.1%승홍용액소독5 min,무균수충세4차~6차,접충도배양기상。재접충8 d~10 d후외식체분화출대유일편소협적신생지。절취자협화하배축작위외식체접충B5+자당30 g/L적배양기중,10 d후형성증식묘。통과조직배양기술,축단향춘적생장주기,재장향춘적눈아주여하처리침포(료액비1∶4,0.05%탄산납)、청세、침탕(95℃수욕2 min,500 mg/kg적포도당산자용액)、력간냉각、절쇄、반료、가입첨가제、장관、살균(85℃,30 min)、냉각、저장。
This topic is based on the seeds of wild toon were cultured as explants, the toon seeds with 75%alcohol solution to disinfect 20 s, and then 0.1%mercuric chloride solution In disinfection 5min, the sterile water 4 to 6 times, inoculated into the medium. Explants differentiation after inoculated 8 d-10 d, with a lobular new branches. Cut to take cotyledon and hypocotyl as explants inoculated B5+sucrose 30 g/L medium, after 10 days form the proliferation of seedlings. By tissue culture techniques to shorten the growth cycle of the toon , and then toon buds do the following processing immersion (1∶4,0.05%sodium carbonate)-Cleaning-scalding liquid ratio (95℃water bath for 2 min, 500 mg/kgglucose solution of zinc)-drain the cooling-chopped-spices-mix in additives-canning-sterilization (85 ° C, 30 min)-cooling-storage.