CAJ | 학술논문

优选棕笋中酚酸类化合物的最佳提取工艺及体外抗氧化活性评价。在单因素试验的基础上，以总酚酸和没食子酸、原儿茶醛、原儿茶酸和咖啡酸提取率为指标，采用正交试验方法，考察料液比、乙醇浓度、提取时间、提取温度等因素对棕笋中酚酸类物质提取率的影响；以清除DPPH·，·OH，以及络合Fe2+能力，综合评价其抗氧化作用。最佳提取工艺为10倍量的50%（体积分数）乙醇，50℃水浴下浸提2次，每次30 min；体外抗氧化实验结果显示其具有较强的体外抗氧化能力，其清除DPPH·，·OH，以及络合Fe2+的IC50分别为0.69、0.56、0.84 mg/mL。优选出的工艺稳定，重复性好，为提取棕笋中酚酸类物质成分提供了实验依据，其具有良好的抗氧剂开发潜力。
우선종순중분산류화합물적최가제취공예급체외항양화활성평개。재단인소시험적기출상，이총분산화몰식자산、원인다철、원인다산화가배산제취솔위지표，채용정교시험방법，고찰료액비、을순농도、제취시간、제취온도등인소대종순중분산류물질제취솔적영향；이청제DPPH·，·OH，이급락합Fe2+능력，종합평개기항양화작용。최가제취공예위10배량적50%（체적분수）을순，50℃수욕하침제2차，매차30 min；체외항양화실험결과현시기구유교강적체외항양화능력，기청제DPPH·，·OH，이급락합Fe2+적IC50분별위0.69、0.56、0.84 mg/mL。우선출적공예은정，중복성호，위제취종순중분산류물질성분제공료실험의거，기구유량호적항양제개발잠력。
To optimize the best process of extracting and the evaluate the antioxidant activities of the phenolic acids from the buds of Trachycarpus fortunei. The influential factors were extraction temperature, ethanol concentration, reflux time and solvent volume fold. The evaluating indicator was the extraction rate of phenolic acids from the buds of T. fortunei by orthogonal test;different antioxidant assays (OH·, DPPH scavenging activity and metal chelating activity) were employed to evaluate the antioxidant activities. The optimum extraction process was 10 volume folds of 50%ethanol, 50℃water bath, and extracting two times, each time 30 min. And have strong antioxidant activity, the IC50 of clearing DPPH and OH, chelating Fe2+was 0.69, 0.56, 0.84 mg/mL, respectively. The process is stable, reproducible, and could be used to extract the phenolic acids from the buds of T. fortunei. And can be a good potential antioxidant.