CAJ | 학술논문

以长白山松子分离蛋白为原料，采用碱性蛋白酶制备抗氧化肽，通过Box-Behnken中心组合试验确定最佳水解工艺条件为：酶解温度56.5℃、加酶量7822 U/g、底物质量浓度0.021 g/mL、pH 9.0、酶解时间240 min。对该条件制备的抗氧化肽进行活性研究，结果显示随着浓度的升高，松子抗氧化肽在4 mg/mL时对ABTS自由基的清除率和Fe2+螯合率达到100%，16 mg/mL时对DPPH自由基清除率达到80.95%，24 mg/mL时，对羟基自由基清除率达到100%。
이장백산송자분리단백위원료，채용감성단백매제비항양화태，통과Box-Behnken중심조합시험학정최가수해공예조건위：매해온도56.5℃、가매량7822 U/g、저물질량농도0.021 g/mL、pH 9.0、매해시간240 min。대해조건제비적항양화태진행활성연구，결과현시수착농도적승고，송자항양화태재4 mg/mL시대ABTS자유기적청제솔화Fe2+오합솔체도100%，16 mg/mL시대DPPH자유기청제솔체도80.95%，24 mg/mL시，대간기자유기청제솔체도100%。
In this experiment, pine nut protein isolated was used as raw material, hydrolyzed by Alcalase to prepare antioxidant peptide.Through Box-Behnken method, the optimal hydrolysis conditions were determined as hydrolysis temperature of 56.5℃,Alcalase concentration of 7 822 U/g, substrate mass concentration of 0.021 g/mL, hydrolysis pH of 9 and hydrolysis time of 240 min. A ssess the antioxidant potential, with the increasing of the peptide solution concentration, ferrous ions chelating capacity and scavenging capacities against ABTS were 100%at 4 mg/mL, scavenging capacities against DPPH was 80.95%at 16 mg/mL, scavenging capacities against hydroxyl radical was 100%at 24 mg/mL.