CAJ | 학술논문

本试验以地榆水煎液为试验药物，以标准金黄色葡萄球菌、无乳链球菌、大肠杆菌为试验菌株，采用琼脂平板扩散法(打孔法)、液体培养基连续稀释法(试管法)和固体培养基连续稀释法，观察中药地榆对奶牛乳房炎常见病原菌抑菌效果的影响。结果表明，药物固体培养基连续稀释法比其他两种方法敏感性高，重复性好，能直接定量测出抗菌药物对奶牛乳房炎常见病原菌的最低MIC值，更适于中药体外抑菌作用的研究。
본시험이지유수전액위시험약물，이표준금황색포도구균、무유련구균、대장간균위시험균주，채용경지평판확산법(타공법)、액체배양기련속희석법(시관법)화고체배양기련속희석법，관찰중약지유대내우유방염상견병원균억균효과적영향。결과표명，약물고체배양기련속희석법비기타량충방법민감성고，중복성호，능직접정량측출항균약물대내우유방염상견병원균적최저MIC치，경괄우중약체외억균작용적연구。
Three different in-vitro bacteriostatic experiment methods were used to determine the minimal inhibitory concentration of sanguisorba on common pathogenic bacterias of dairy cow mastitis. Sanguisorba water decoction were used as experimental medicine, the experimental bacterial strain were standard Staphylococcus aureus, Streptococcus agalactiae and E.coli. The agar plate diffusion method (play), liquid medium serial dilution method (test tube) and solid medium serial dilution method were used to observe TCM bacteriostatic effect. Results showed that Solid medium serial dilution method had higher sensitivity and better repeatability. Solid medium serial dilution method was more suitable to study the in-vitro anti-bacterial effect of TCM for it could directly measure the common pathogenic bacterial MIC values.