CAJ | 학술논문

试验从健康母猪粪便中分离乳酸菌，并对其益生特性进行初步研究。通过形态和生理生化鉴定，再利用16S rDNA序列同源性分析的方法进行16S rDNA基因扩增与测序，并将测序结果与GenBank中菌株的16S rDNA基因序列进行同源性比较分析，得到1株链球菌，2株约氏乳杆菌，4株乳酸片球菌。体外益生特性结果表明，各菌株在pH 2.0～3.0，胆盐浓度0.1%～0.3%均有较强的耐受能力，各菌株对大肠埃希氏菌和单核细胞增多症李氏杆菌均有较好的抑制作用。
시험종건강모저분편중분리유산균，병대기익생특성진행초보연구。통과형태화생리생화감정，재이용16S rDNA서렬동원성분석적방법진행16S rDNA기인확증여측서，병장측서결과여GenBank중균주적16S rDNA기인서렬진행동원성비교분석，득도1주련구균，2주약씨유간균，4주유산편구균。체외익생특성결과표명，각균주재pH 2.0～3.0，담염농도0.1%～0.3%균유교강적내수능력，각균주대대장애희씨균화단핵세포증다증리씨간균균유교호적억제작용。
In order to gain the lactic acid bacteria as the probiotics and explore the probiotic characteristics,lactic acid bacteria strains were isolated from the sow feces.The morphological and physiological and biochemical identification were determined.Then the genes of 16S rDNA of these strains were amplified and sequenced using the 16S rDNA se-quence homology analysis.The sequence results of these strains were compared with other 16S rDNA sequences in Gen-Bank.By Blast assay,1 strain was Streptococcus sp.,2 strains were Lactobacillus johnsonii,and 4 strains were Pediococcus acidilactici.The results showed that probiotic characteristics in vitro showed all strains had higher tolerance to the pH 2.0 ~ 3.0 and bile concentrations in range of 0.1% ~ 0.3% and all strains had better inhibitory effect to Escherichia coli and Listeria monocytogenes.