中国民族民间医药
中國民族民間醫藥
중국민족민간의약
CHINESE JOURNAL OF ETHNOMEDICINE AND ETHNOPHARMACY
2014年
14期
22-23,25
,共3页
曾琦%齐元博%薛锦文%王俊影%聂发玉%张象涵
曾琦%齊元博%薛錦文%王俊影%聶髮玉%張象涵
증기%제원박%설금문%왕준영%섭발옥%장상함
鸡骨草%毛鸡骨草%指纹图谱%HPLC
鷄骨草%毛鷄骨草%指紋圖譜%HPLC
계골초%모계골초%지문도보%HPLC
Abrus cantoniensis%Abrus Mollis%fingerprint chromatogram%HPLC
目的:建立鸡骨草和毛鸡骨草两种药材的化学指纹图谱。方法:采用HPLC高效液相色谱法,色谱柱Inertsil ODS-SP C18(4.6×250mm,5μm),水-甲醇10%~100%梯度洗脱40min,流速1.0mL/min,柱温25℃,检测波长280 nm。结果:分别建立了鸡骨草和毛鸡骨草的指纹图谱,显示出特征峰。结论:HPLC指纹图谱中各峰分离度较好,方法具有良好的精密度、重复性和稳定性,为鸡骨草和毛鸡骨草的化学有效成分的异同及替代安全性问题研究提供参考。
目的:建立鷄骨草和毛鷄骨草兩種藥材的化學指紋圖譜。方法:採用HPLC高效液相色譜法,色譜柱Inertsil ODS-SP C18(4.6×250mm,5μm),水-甲醇10%~100%梯度洗脫40min,流速1.0mL/min,柱溫25℃,檢測波長280 nm。結果:分彆建立瞭鷄骨草和毛鷄骨草的指紋圖譜,顯示齣特徵峰。結論:HPLC指紋圖譜中各峰分離度較好,方法具有良好的精密度、重複性和穩定性,為鷄骨草和毛鷄骨草的化學有效成分的異同及替代安全性問題研究提供參攷。
목적:건립계골초화모계골초량충약재적화학지문도보。방법:채용HPLC고효액상색보법,색보주Inertsil ODS-SP C18(4.6×250mm,5μm),수-갑순10%~100%제도세탈40min,류속1.0mL/min,주온25℃,검측파장280 nm。결과:분별건립료계골초화모계골초적지문도보,현시출특정봉。결론:HPLC지문도보중각봉분리도교호,방법구유량호적정밀도、중복성화은정성,위계골초화모계골초적화학유효성분적이동급체대안전성문제연구제공삼고。
Objective To establish a HPLC fingerprint analysis method for Abrus cantoniensis and Abrus Mollis.Methods Inertsil ODS-SP column (250mm ×4.6mm,5μm)was used,with gradient elution by the mobile phase consisted of water-Methanol 10-100%.The flow rate was 1.0 mL/min,and the detection wavelength was 210 nm.Results The offingerprint of A.cantoniensis and A.Mollis were established,which show some common peaks between them.Conclusion The characteristic peaks are selected with good resolution,and the method is accurate and stable.It can be used for the study of the similarities and differences of chemical ac-tive ingredients between A.cantoniensis and A.Mollis.