CAJ | 학술논문

目的：探讨石菖蒲挥发油对氯化锂-匹鲁卡品致痫大鼠行为学改善的抗氧化机制。方法将成年雄性SD大鼠66只随机分为对照组（6只）、癫痫组（30只）、石菖蒲挥发油组（30只），后两组采用氯化锂-匹鲁卡品法建立癫痫动物模型；观察各组行为学变化。癫痫模型大鼠在致痫后4、24、48 h断头取脑，检测海马组织中丙二醛（MDA）、一氧化氮（NO）、超氧化物歧化酶（SOD）的含量。结果（1）行为学：癫痫组与石菖蒲挥发油组首达4级发作时间潜伏期比较，差异有统计学意义（P<0.05）。（2）生化指标改变：癫痫组较石菖蒲挥发油组MDA、NO含量明显升高、SOD含量明显下降，差异均有统计学意义（P<0.05），癫痫组和石菖蒲挥发油组的自由基变化与对照组比较，差异均有统计学意义（P<0.05）。结论石菖蒲挥发油能改善氯化锂-匹鲁卡品致痫大鼠的行为，并可能通过抑制MDA、NO含量的增加和SOD的减少，保护海马区神经元损伤。
목적：탐토석창포휘발유대록화리-필로잡품치간대서행위학개선적항양화궤제。방법장성년웅성SD대서66지수궤분위대조조（6지）、전간조（30지）、석창포휘발유조（30지），후량조채용록화리-필로잡품법건립전간동물모형；관찰각조행위학변화。전간모형대서재치간후4、24、48 h단두취뇌，검측해마조직중병이철（MDA）、일양화담（NO）、초양화물기화매（SOD）적함량。결과（1）행위학：전간조여석창포휘발유조수체4급발작시간잠복기비교，차이유통계학의의（P<0.05）。（2）생화지표개변：전간조교석창포휘발유조MDA、NO함량명현승고、SOD함량명현하강，차이균유통계학의의（P<0.05），전간조화석창포휘발유조적자유기변화여대조조비교，차이균유통계학의의（P<0.05）。결론석창포휘발유능개선록화리-필로잡품치간대서적행위，병가능통과억제MDA、NO함량적증가화SOD적감소，보호해마구신경원손상。
Objective To investigate the antioxidative mechanism of Acorus tatarinowii Schott volatile oil on the behav-ioral improvement of epilepsy rats induced by lithium-pilocarpine. Methods Sixty-six adult male SD rats were randomly divided into control group (n=6),epilepsy group (n=30),volatile oil of Acorus tatarinowii Schott group (n=30),and the rat models of the latter two groups were established with lithium-pilocarpine method;the behavioral changes of all the groups were observed. The rats in the epilepsy group were decapitated at the 4th,24th and 48th h after epilepsy,to detect the content of methane dicarboxylic aldehyde (MAD),nitric oxide (NO) and superoxide dismutase (SOD) in hippocampus. Results (1)The behavioristics showed that the comparison of incubation period between the epilepsy group and volatile oil of Acorus tatarinowii Schott group reached to level 4 had statistically significant difference(P<0.05). (2)The changes of biochemical indexes manifested that the contents of MDA and NO in the epilepsy group and volatile oil of Acorus tatarinowii Schott group increased obviously ,while the contents of SOD decreased prominently,and the differences between the two groups had statistical significance (P<0.05);compared with the control group,the free radical changes in the epilepsy group and volatile oil of Acorus tatarinowii Schott group had statistically significant difference(P<0.05). Conclusion The volatile oil of Acorus tatarinowii Schott can improve the behavior of epilepsy rates induced by lithium-Pilocorpine and inhibit the content of MDA,NO and SOD,so it is involved in the protection of neuronal damage of hippocampus.