CAJ | 학술논문

目的探讨电针治疗对骨癌痛吗啡耐受模型大鼠痛行为的影响以及背根神经节降钙素基因相关肽(CGRP)表达的变化.方法 40只SD大鼠随机分为4组,每组10只:假手术组(Sham)、骨癌痛+吗啡耐受组(BM)、骨癌痛+电针组(BE)和骨癌痛+吗啡耐受+电针组(BME).BM、BE和BME组制备胫骨癌痛模型,接种后第7天3组分别实施吗啡、电针、吗啡+电针治疗,连续9d.电针选择足三里(ST36)和三阴交(SP6)穴位.痛行为采用机械刺激缩足阈值测定,以50％缩足阈表示.免疫组织化学测定背根神经节CGRP表达水平.结果电针治疗第5天,BME组50％缩足阈值(10.9±0.8)g,与BM组[(8.7±0.6)g]和BE组[(6.2±0.9)g]相比,差异有统计学意义(P＜0.01),并持续至第9天.BME组背根神经节CGRP免疫阳性表达的IOD值(9026.5±1827.4),与BM组(14803.1±2086.7)和BE组(15730.6±2712.5)比较,差异有统计学意义(P＜0.01).结论电针能够改善骨癌痛-吗啡耐受大鼠机械痛阈,机制可能与减少背根神经节CGRP过度释放有关.
목적 탐토전침치료대골암통마배내수모형대서통행위적영향이급배근신경절강개소기인상관태(CGRP)표체적변화.방법 40지SD대서수궤분위4조,매조10지:가수술조(Sham)、골암통+마배내수조(BM)、골암통+전침조(BE)화골암통+마배내수+전침조(BME).BM、BE화BME조제비경골암통모형,접충후제7천3조분별실시마배、전침、마배+전침치료,련속9d.전침선택족삼리(ST36)화삼음교(SP6)혈위.통행위채용궤계자격축족역치측정,이50％축족역표시.면역조직화학측정배근신경절CGRP표체수평.결과 전침치료제5천,BME조50％축족역치(10.9±0.8)g,여BM조[(8.7±0.6)g]화BE조[(6.2±0.9)g]상비,차이유통계학의의(P＜0.01),병지속지제9천.BME조배근신경절CGRP면역양성표체적IOD치(9026.5±1827.4),여BM조(14803.1±2086.7)화BE조(15730.6±2712.5)비교,차이유통계학의의(P＜0.01).결론 전침능구개선골암통-마배내수대서궤계통역,궤제가능여감소배근신경절CGRP과도석방유관.
Objective To explore the efficacy of electroacupuncture treatment in cancer induced bone pain (CIBP)-morphine tolerance rat model and the expression of calcitonin-gene related peptide(CGRP) immunohistochemisty in dorsal root ganglion (DRG).Methods Forty SD rats were divided into four groups:sham group,CIBP + morphine tolerance group (BM),CIBP + electroacupuncture group (BE),and CIBP + morphine tolerance + electroacupuncture group (BME).BM,BE and BME groups were prepared CIBP model by carcinoma cell tibia implanted.After six days,the three CIBP groups accepted treatment of morphine,electroacupuncture,and morphine combined electroacupuncture,separately,nine days continuously.Acupoints were selected Zusanli (ST36) and Sanyinjiao (SP6).50％ mechanical withdraw threshold was evaluated by mechanical stimulation.CGRP expression in dorsal root ganglion was detected by immunohistochemisty.Results After five days of electroacupuncture treatment,pain threshold was (10.9 ± 0.8) g in BME group,(8.7 ± 0.6) g in BM group and (6.2 ± 0.9) g in BE group.The results had significant statistic differences (P ＜ 0.01,separately).IOD value of CGRP expression in dorsal root ganglion was 9026.5 ± 1827.4 in BME group,compared with 14803.1 ± 2086.7 in BM group and 15730.6 ± 2712.5 in BE group (P ＜ 0.01,separately).Results Electroacupuncture can relieve CIBP-morphine tolerance rat pain behavior.The mechanism is related to inhibiting CGRP overdue expression in DRG.