中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2013年
5期
391-393
,共3页
脊髓损伤%神经行为%病理
脊髓損傷%神經行為%病理
척수손상%신경행위%병리
Spinal cord injury%Neurobehavior%Pathophysiology
目的 观察电控打击脊髓损伤(spinal cord injury,SCI)模型大鼠神经行为及脊髓病理变化.方法 采用自行研制的电控大鼠脊髓损伤打击器建立大鼠脊髓损伤模型,观察脊髓损伤后Basso Beattie Bresnahan (BBB)评分、免疫组化及病理变化特点.结果 SCI后3 d BBB评分(1.0±0.7)开始增加,7d时显著明显增加至(4.2±1.3)分,28 d时BBB评分达(7.2±1.3)分.HE染色可观察到SCI后脊髓灰、白质组织结构不完整,大量神经元变性坏死、细胞肿胀、胶质细胞增生.免疫组化显示SCI伤后2h神经元特异性烯醇化酶(NSE)阳性细胞灰度值、神经丝蛋白-200(NF200)阳性轴突灰度值减少,24 h最低,3d开始恢复,28 d仍未恢复正常(P＜0.05)；SCI伤后3d胶质纤维酸性蛋白(GFAP)阳性细胞数量增多,14 d达到高峰,28 d有所回落(P＜0.05).NSE、NF200、GFAP的变化与BBB评分呈显著相关(r=0.856,0.856,0.795,均P＜0.01).结论 电控打击脊髓损伤模型大鼠NSE、NF、GFAP的变化反映了脊髓损伤的病理变化,与脊髓损伤后的神经行为密切相关.
目的 觀察電控打擊脊髓損傷(spinal cord injury,SCI)模型大鼠神經行為及脊髓病理變化.方法 採用自行研製的電控大鼠脊髓損傷打擊器建立大鼠脊髓損傷模型,觀察脊髓損傷後Basso Beattie Bresnahan (BBB)評分、免疫組化及病理變化特點.結果 SCI後3 d BBB評分(1.0±0.7)開始增加,7d時顯著明顯增加至(4.2±1.3)分,28 d時BBB評分達(7.2±1.3)分.HE染色可觀察到SCI後脊髓灰、白質組織結構不完整,大量神經元變性壞死、細胞腫脹、膠質細胞增生.免疫組化顯示SCI傷後2h神經元特異性烯醇化酶(NSE)暘性細胞灰度值、神經絲蛋白-200(NF200)暘性軸突灰度值減少,24 h最低,3d開始恢複,28 d仍未恢複正常(P＜0.05)；SCI傷後3d膠質纖維痠性蛋白(GFAP)暘性細胞數量增多,14 d達到高峰,28 d有所迴落(P＜0.05).NSE、NF200、GFAP的變化與BBB評分呈顯著相關(r=0.856,0.856,0.795,均P＜0.01).結論 電控打擊脊髓損傷模型大鼠NSE、NF、GFAP的變化反映瞭脊髓損傷的病理變化,與脊髓損傷後的神經行為密切相關.
목적 관찰전공타격척수손상(spinal cord injury,SCI)모형대서신경행위급척수병리변화.방법 채용자행연제적전공대서척수손상타격기건립대서척수손상모형,관찰척수손상후Basso Beattie Bresnahan (BBB)평분、면역조화급병리변화특점.결과 SCI후3 d BBB평분(1.0±0.7)개시증가,7d시현저명현증가지(4.2±1.3)분,28 d시BBB평분체(7.2±1.3)분.HE염색가관찰도SCI후척수회、백질조직결구불완정,대량신경원변성배사、세포종창、효질세포증생.면역조화현시SCI상후2h신경원특이성희순화매(NSE)양성세포회도치、신경사단백-200(NF200)양성축돌회도치감소,24 h최저,3d개시회복,28 d잉미회복정상(P＜0.05)；SCI상후3d효질섬유산성단백(GFAP)양성세포수량증다,14 d체도고봉,28 d유소회락(P＜0.05).NSE、NF200、GFAP적변화여BBB평분정현저상관(r=0.856,0.856,0.795,균P＜0.01).결론 전공타격척수손상모형대서NSE、NF、GFAP적변화반영료척수손상적병리변화,여척수손상후적신경행위밀절상관.
Objective To observe neurobehavior and pathological changes of spinal cord injury (SCI) model in adult rats.Methods The Basso Beattie Bresnahan (BBB) scoring,histological and immunohistochemical changes after SCI were observed by means of self-designed spinal cord injurying device controlled by electrocircuit.Results From the 3rd day(1.0 ± 0.7),the BBB score of injured group began to increase (increased to 4.2 ± 1.3significantly at 7th day,and at the 28th day was 7.2 ± 1.3),and the hind limb could not support the body weight.Degeneration,necrosis of lots of neurons and glial cells proliferation could be observed obviously in spinal cord injured group.Immunohistochemical experiments showed that the grey level began to decrease 2nd hour after injury,went down the minimum at the 1st day,then 3rd day recovered,and at 28 th day was still lower than that of sham group.The glial fibrillary acidic protein (GFAP) immunostaining positive cells area was gradually increased from 3rd day,reached the apex in the 14 th day,then decreased in the 28 th day.The changes of neuron specific enolase (NSE),neurofilament protein 200 (NF200) and GFAP had a close relationship with BBB score (r =0.856,0.856,0.795 respectively,P＜0.01).Conclusion The changes of NSE,NF200,GFAP after spinal cord injury reflect the characteristic of pathological change,which have a close relationship with functional recovery.
