CAJ | 학술논문

目的：本研究旨在探索Th17细胞分泌的标志性细胞因子IL-17在口腔扁平苔藓（OLP）局部病变中的作用机制。方法：24例网状型OLP患者、19例萎缩糜烂型OLP患者和13位健康志愿者被募集参与本实验。采用实时定量PCR实验检测IL-17 mRNA和CCL20 mRNA在OLP组织中的表达情况。结果：网状型OLP组和萎缩糜烂型OLP组口腔黏膜病损组织中IL-17基因的表达显著高于正常口腔黏膜组织（P＝0．0095，P＜0．0001），同时其在萎缩糜烂型OLP组组织中的表达量明显高于网状型OLP组（P＝0．0012）。 CCL20基因在网状型OLP组和萎缩糜烂型OLP组局部组织中的表达显著高于对照组（P＝0．0357，P＜0．0001）；在萎缩糜烂型OLP患者局部组织中的表达较网状型OLP组升高明显。而且OLP患者病损组织中CCL20 mRNA的表达随IL-17 mRNA的表达升高而增加，IL-17与病损局部的CCL20的产生呈正相关（P＝0．003）。结论：IL-17可诱导角化细胞释放趋化因子CCL20，这一信号通路可能是促进相关炎症细胞向OLP局部病损组织迁徙和浸润的重要途径。
목적：본연구지재탐색Th17세포분비적표지성세포인자IL-17재구강편평태선（OLP）국부병변중적작용궤제。방법：24례망상형OLP환자、19례위축미란형OLP환자화13위건강지원자피모집삼여본실험。채용실시정량PCR실험검측IL-17 mRNA화CCL20 mRNA재OLP조직중적표체정황。결과：망상형OLP조화위축미란형OLP조구강점막병손조직중IL-17기인적표체현저고우정상구강점막조직（P＝0．0095，P＜0．0001），동시기재위축미란형OLP조조직중적표체량명현고우망상형OLP조（P＝0．0012）。 CCL20기인재망상형OLP조화위축미란형OLP조국부조직중적표체현저고우대조조（P＝0．0357，P＜0．0001）；재위축미란형OLP환자국부조직중적표체교망상형OLP조승고명현。이차OLP환자병손조직중CCL20 mRNA적표체수IL-17 mRNA적표체승고이증가，IL-17여병손국부적CCL20적산생정정상관（P＝0．003）。결론：IL-17가유도각화세포석방추화인자CCL20，저일신호통로가능시촉진상관염증세포향OLP국부병손조직천사화침윤적중요도경。
Objective To investigate the mechanism of IL-17, the signature cytokine produced by Th17 cells, in OLP lesion. Methods 24 patients with reticular OLP, 19 patients with atrophic-erosive OLP and 13 healthy volunteers were enrolled in this study . Real-time quantitative PCR ( real-time qPCR ) was performed to analyze the expressions of the production of IL-17 and CCL20 mRNA. Results The expressions of IL-17 mRNA in reticular OLP and atrophic-erosive OLP were significant higher than that in healthy oral mucosa (P = 0.0095, P <0.0001, respectively), meanwhile, remarkable increased IL-17 expression in atrophic-erosive OLP group was found compared with reticular OLP group (P = 0.0012). Additionally, the expressions of CCL20 mRNA in reticular OLP and atrophic-erosive OLP were significant higher than that in control group (P=0.0357, P<0.0001, respectively), meanwhile, CCL20 expression in atrophic-erosive OLP was higher than that in reticular OLP. The expressions of CCL20 mRNA rises with the increased expression of IL-17, and were positive correlated with IL-17 expressions in OLP lesions (P=0.003). Conclusions IL-17 production can induce chemokine CCL20 expression in OLP lesion. The signal pathway may promote the migration and infiltration of inflammatory cells in OLP lesions.