食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2014年
14期
87-90
,共4页
韩镌竹%李欣南%苏崴艺%于雷
韓鐫竹%李訢南%囌崴藝%于雷
한전죽%리흔남%소외예%우뢰
羊肉%多重PCR-RFLP%掺假检测
羊肉%多重PCR-RFLP%摻假檢測
양육%다중PCR-RFLP%참가검측
mutton%duplex PCR-RFLP%adulteration detection
以纯羊肉为原料，按2%、5%和10%的重量比在羊肉中掺杂鸡肉、猪肉和鼠肉，通过基因组DNA提取试剂盒法提取DNA，利用多重多聚酶链反应（Polymerase Chain Reaction ，PCR）及限制性片段长度多态性（Restriction Fragment Length Polymorphism， RFLP）分析同时检测羊肉及掺杂其中的其他肉类。结果表明，通过多重PCR扩增，可以有效的检测出纯羊肉及其掺杂的鸡肉、猪肉和鼠肉，检出限为2%。
以純羊肉為原料，按2%、5%和10%的重量比在羊肉中摻雜鷄肉、豬肉和鼠肉，通過基因組DNA提取試劑盒法提取DNA，利用多重多聚酶鏈反應（Polymerase Chain Reaction ，PCR）及限製性片段長度多態性（Restriction Fragment Length Polymorphism， RFLP）分析同時檢測羊肉及摻雜其中的其他肉類。結果錶明，通過多重PCR擴增，可以有效的檢測齣純羊肉及其摻雜的鷄肉、豬肉和鼠肉，檢齣限為2%。
이순양육위원료，안2%、5%화10%적중량비재양육중참잡계육、저육화서육，통과기인조DNA제취시제합법제취DNA，이용다중다취매련반응（Polymerase Chain Reaction ，PCR）급한제성편단장도다태성（Restriction Fragment Length Polymorphism， RFLP）분석동시검측양육급참잡기중적기타육류。결과표명，통과다중PCR확증，가이유효적검측출순양육급기참잡적계육、저육화서육，검출한위2%。
Pure mutton or the mixture with 2%, 5%and 10%chicken, pork and mouse meat were studied. Genomic DNA was extracted with t DNA extraction kit. Pure mutton and the mixtures were detected simultaneously by duplex polymerase chain reaction(PCR) and restriction fragment length polymorphism(RFLP) techniques. The results showed that method introduced in this study could detect pure mutton , chicken, pork or mouse meat adulterated in it effectively. The detection limitation was 2%.
