CAJ | 학술논문

用计算机分子模拟对功能单体和模板分子间的结合作用进行了研究，选择结合能最强的甲基丙烯酸作为功能单体。以γ-(甲基丙烯酰氧)丙基三甲氧基硅烷(MPS)为媒介，将功能单体甲基丙烯酸(MAA)接枝于SiO2微粒表面，制得了功能接枝微粒 PMAA/SiO2。以PMAA/SiO2为载体，甲基丙烯酰胺(MAA)为功能单体，乙二醇二甲基丙烯酸酯(EDMA)为交联剂，采用溶液聚合法制备了以胸腺五肽为模板分子的表面印迹微粒(Tp5-MIP)。分别通过动态吸附实验和等温吸附实验研究了该印迹微粒对胸腺五肽的识别特性。结果表明，该印迹微粒对印迹分子具有较好的亲和能力及选择性，其识别能力来自于印迹得到的识别位点。
용계산궤분자모의대공능단체화모판분자간적결합작용진행료연구，선택결합능최강적갑기병희산작위공능단체。이γ-(갑기병희선양)병기삼갑양기규완(MPS)위매개，장공능단체갑기병희산(MAA)접지우SiO2미립표면，제득료공능접지미립 PMAA/SiO2。이PMAA/SiO2위재체，갑기병희선알(MAA)위공능단체，을이순이갑기병희산지(EDMA)위교련제，채용용액취합법제비료이흉선오태위모판분자적표면인적미립(Tp5-MIP)。분별통과동태흡부실험화등온흡부실험연구료해인적미립대흉선오태적식별특성。결과표명，해인적미립대인적분자구유교호적친화능력급선택성，기식별능력래자우인적득도적식별위점。
The functional monomer of methylacrylic acid (MAA)and acrylamide (AM)were evaluated using computer simulation technological,the MAA was selected as functional monomer.By coupling action of 3-methacryloxypropyl trimethoxysilane (MPS),polymethylacrylic acid (PMAA)was grafted on the surface of silica particles.Using solution polymerization method,the molecular imprinted polymer Tp5-MIP was obtained using PMAA/SiO2 as vector,thymopentin as the template molecular and ethylene glycol dimethacrylate (ED-MA)as the crosslinking agent.Both static and dynamic binding properties of Tp5-MIP for thymopentin were studied.Result shows that Tp5-MIP exhibits excellent binding affinity and specific selectivity for the template molecule.The recognition resulted from the imprinting process.