CAJ | 학술논문

基于PLLA(左旋聚乳酸)的优异性能,以六氟异丙醇(HFIP)为溶剂采用静电纺丝法制备质量比为0∶100,30∶70,50∶50,70∶30和100∶0的8%SF/COL/PLLA共混纳米纤维支架,并用浓度为25%戊二醛蒸汽交联48 h,通过扫描电镜、X 射线衍射、热重分析仪、接触角检测仪对共混材料交联前后的理化性能进行表征。结果表明纳米纤维直径均一,分布均匀；交联后Silk I 形成稳定的β折叠,结晶度增强,且交联后材料结晶度明显较交联前增强；热稳定性也随着PLLA浓度的增加愈加稳定。交联后的材料接触角均大于90°表明材料为疏水性,PLLA的加入降低了材料的亲水性能。复合人牙周膜干细胞和材料培养,分析材料的生物学性能,结果证明细胞培养5,7和9 d时,细胞在不同比例的支架上粘附、铺展和增殖均良好,但细胞与质量比为30∶70的 SF/COL/PLCL 纤维膜复合生长最好。
기우PLLA(좌선취유산)적우이성능,이륙불이병순(HFIP)위용제채용정전방사법제비질량비위0∶100,30∶70,50∶50,70∶30화100∶0적8%SF/COL/PLLA공혼납미섬유지가,병용농도위25%무이철증기교련48 h,통과소묘전경、X 사선연사、열중분석의、접촉각검측의대공혼재료교련전후적이화성능진행표정。결과표명납미섬유직경균일,분포균균；교련후Silk I 형성은정적β절첩,결정도증강,차교련후재료결정도명현교교련전증강；열은정성야수착PLLA농도적증가유가은정。교련후적재료접촉각균대우90°표명재료위소수성,PLLA적가입강저료재료적친수성능。복합인아주막간세포화재료배양,분석재료적생물학성능,결과증명세포배양5,7화9 d시,세포재불동비례적지가상점부、포전화증식균량호,단세포여질량비위30∶70적 SF/COL/PLCL 섬유막복합생장최호。
The aim of this study was to prepare a kind of nanofiber scaffolds composed of different mass ratios of silkfi-broin(SF)/collagen/PLLA by using electrospinning technique and to evaluate its properties.1 ,1 ,1 ,3 ,3 ,3-hexafluoro-2-propanol (HFIP)was taken as solvent to prepare the nanofiber scaffolds and the mass ratios of SF/collagen/PLLA were set as 0∶100,30∶70,50∶50,70∶30,100∶0.And then the preoperties of the as-prepared samples were tested before and after crosslinked by 25% glutaraldehyde vapor for 48 h.The scaffolds were characterized by scanning electron mi-croscopy (SEM),X-ray diffraction (XRD),thermo gravimetric (TG)analysis and the hydrophily was analysed by water contact angle (WCA)analysis.The results of SEM revealed that the uniformity of the blending nanofibers had im-proved.The thermal stability of the scaffold material increased with the increase of the blend ratios of PLLA.The XRD results indicated that stableβ-folding conformation of silk has formed and the crystallization of materials has increased. The contact water angle meter analysis suggested that the hydrophilic property of the blending composite nanofibers de-creased with the existence of PLLA.The periodontal ligament stem cells (PDLSCs)were seeded on the blending com-posite nanofibers in vitro.The cells grew well on the surface of materials,and had great adhesion and proliferation abili-ties.The cytocompatibility and physicochemical properties of the scaffold with content of 70% PLLA are superior to other groups,which suggest the as-prepared sampleis expected to become a new type of scaffold forperiodontal tissue en-gineering.