CAJ | 학술논문

壳聚糖及其衍生物作为生物医用高分子材料已被广泛应用，研究其在动物体内的吸收降解是此类生物医用材料应用于人体的必要前提。壳聚糖即型水凝胶是以壳聚糖为主要材料制备的新型生物医用材料，可在体内降解吸收。以异硫氰酸酯(FITC)标记法研究了该水凝胶经大鼠腹腔注射后的吸收、降解和分布，为该水凝胶应用于体内提供实验数据。实验结果显示，壳聚糖即型水凝胶经大鼠腹腔注射后能够较快被腹腔吸收，并经血液转运至各代谢器官，在肝脏内有一过性的积累，在其它脏器内分布较少，其主要代谢途径是血液→肝脏→肾脏→尿液，在17 d 时，通过此途径排出的量占注射量的88%，排出体外的分子量均小于10 kD。
각취당급기연생물작위생물의용고분자재료이피엄범응용，연구기재동물체내적흡수강해시차류생물의용재료응용우인체적필요전제。각취당즉형수응효시이각취당위주요재료제비적신형생물의용재료，가재체내강해흡수。이이류청산지(FITC)표기법연구료해수응효경대서복강주사후적흡수、강해화분포，위해수응효응용우체내제공실험수거。실험결과현시，각취당즉형수응효경대서복강주사후능구교쾌피복강흡수，병경혈액전운지각대사기관，재간장내유일과성적적루，재기타장기내분포교소，기주요대사도경시혈액→간장→신장→뇨액，재17 d 시，통과차도경배출적량점주사량적88%，배출체외적분자량균소우10 kD。
Chitosan based in situ forming hydrogel(ChG)was composed of chitosan derivative and oxidized algi-nate which was designed as a hemostasis in the operation.It was of critical importance to study the pharmacoki-netic features and biodegradation mechanism of this new biomaterial.Here,we present the pharmacokinetic mode of fluorescein isothiocyanate-labeled ChG (FITC-ChG)in rats.It was found that through intraperitoneal administration,FITC-ChG was absorbable,biodegradable and can be excreted rapidly.The main pathway of excretion of FITC-ChG was blood absorption,liver degradation and urinary excretion,88% of the injected gel dose can be excreted in urine over the period of 1 7 d.And the gel permeation chromatography (GPC)analysis indicate that ChG had undergone an obj ective degradation process,the molecular weight of the FITC-ChG can be degraded from over 200 kD to less than 10 kD in 24 h.All the results demonstrate that ChG has excellent bioavailability and biodegradability,which suggests its promising applications in the biomedical area.