CAJ | 학술논문

目的：探讨乳腺癌患者癌细胞 ER、PR、C-erbB-2、P53、Ki67表达的应用价值及质量控制。方法采用细针吸取细胞学涂片或术中细胞学印片获取标本，联合免疫细胞化学染色，研究53例乳腺癌患者癌细胞 ER、PR、C-erbB-2、P53、Ki67的生物学表达，并与免疫组织化学染色进行对照分析。结果53例乳腺癌患者 ER、PR、C-erbB-2、P53、Ki67的生物学表达分别为73．58％、58．49％、56．60％、68．63％、81．13％，ER、PR、C-erbB-2、P53、Ki67阳性病例和阳性强度，免疫细胞化学染色与免疫组织化学染色之间差异均无统计学意义（P ＞0．05）。结论免疫细胞化学染色可替代免疫组织化学染色，在乳腺癌的术前诊断、治疗方案的选择、以及预后的判断具有重要的意义。全过程的质量控制是做好乳腺癌免疫细胞化学染色的关键。
목적：탐토유선암환자암세포 ER、PR、C-erbB-2、P53、Ki67표체적응용개치급질량공제。방법채용세침흡취세포학도편혹술중세포학인편획취표본，연합면역세포화학염색，연구53례유선암환자암세포 ER、PR、C-erbB-2、P53、Ki67적생물학표체，병여면역조직화학염색진행대조분석。결과53례유선암환자 ER、PR、C-erbB-2、P53、Ki67적생물학표체분별위73．58％、58．49％、56．60％、68．63％、81．13％，ER、PR、C-erbB-2、P53、Ki67양성병례화양성강도，면역세포화학염색여면역조직화학염색지간차이균무통계학의의（P ＞0．05）。결론면역세포화학염색가체대면역조직화학염색，재유선암적술전진단、치료방안적선택、이급예후적판단구유중요적의의。전과정적질량공제시주호유선암면역세포화학염색적관건。
Objective To investigate the application value and quality control of breast cancer cells PR,ER C-erbB-2,P53,Ki67 expressions in the patients with breast cancer.Methods The specimens were obtained by the fine needle aspiration cytological smear or intraoperative imprint cytology.The biological expressions of ER,PR,C-erbB-2,P53,Ki67 were detected by the immuno-cytochemical staining,the detection results with those detected by the immunohistochemical staining were performed the compara-tive analysis.Results The biological expressions of ER,PR,C-erbB-2,P53,Ki67 in 53 cases of breast cancer were 73.56%, 58.49%,56.60%,68.63%,81.13% respectively;ER,PR,C-erbB-2,P53,Ki67 had no statistical significant differences in positive cases and positive intensity between the immunocytochemical staining and the immunohistochemical staining (P >0.05).Conclusion The immunocytochemical staining can replace the immunohistochemical staining and has the important significance in the preop-erative diagnosis of breast cancer,therapeutical scheme selection and prognosis judgment.The whole process quality control is the key to do the breast cancer immunocytochemical staining well.