CAJ | 학술논문

目的：以实时荧光定量 PCR（RT-PCR）技术检测耐甲氧西林葡萄球菌（MRSA）mecA 基因为金标准，进行与头孢西丁纸片筛选试验的评价。方法收集各种细菌感染性标本分离到的葡萄球菌，分别采用 PCR 扩增 mecA 法、头孢西丁筛选试验检测 MRSA。结果 RT-PCR 检出金黄色葡萄球菌中携带 mecA 基因占54．55％（66/121）。与 VITEK2细菌鉴定仪头孢西丁筛选试验的结果比较差异无统计学意义（P ＞0．05）。头孢西丁筛选试验敏感度93．93％，特异度81．82％，阳性预期值84．93％，阴性预期值91．83％。结论 RT-PCR 技术可准确、快速检测 MRSA。
목적：이실시형광정량 PCR（RT-PCR）기술검측내갑양서림포도구균（MRSA）mecA 기인위금표준，진행여두포서정지편사선시험적평개。방법수집각충세균감염성표본분리도적포도구균，분별채용 PCR 확증 mecA 법、두포서정사선시험검측 MRSA。결과 RT-PCR 검출금황색포도구균중휴대 mecA 기인점54．55％（66/121）。여 VITEK2세균감정의두포서정사선시험적결과비교차이무통계학의의（P ＞0．05）。두포서정사선시험민감도93．93％，특이도81．82％，양성예기치84．93％，음성예기치91．83％。결론 RT-PCR 기술가준학、쾌속검측 MRSA。
Objective To evaluate the cefoxitin screening test in detecting(MRSA)with the real-time fluorescent quantitative polymerase chain reaction(RT-PCR)for detecting mecA gene as the golden standard.Methods Staphylococcus aureus strains iso-lated from various bacterial infection specimens were collected,which were amplified by RT-PCR and detected by the cefoxitin screening test respectively.The results were compared.Results In 121strains of MRSA,66 strains carrying mecA gene were detec-ted by RT-PCR(P >0.05),accounting for 54.55%(66/121).There was no statsitcal difference in the detection results between the cefoxitin screening test and RT-PCR.The sensitivity of the cefoxitin screening test was 93.93%,the specificity was 81.82%,the positive predictive value was 84.93% and negative predictive value was 91.83%.Conclusion The RT-PCR technique can accurate-ly and rapidly detect MRSA.