CAJ | 학술논문

目的：探讨毛细管电泳在分析糖基化血红蛋白水平方面的临床应用价值。方法该文利用长30 cm、内径25μm 的未涂层熔融石英毛细管，利用血红蛋白在415 nm 波长处的特异性吸收峰，二极管阵列（PDA）法检测健康人与糖尿病及高危人群中的糖基化血红蛋白（HbA1c）水平。在研究过程中，对精密度和准确度进行了精确分析。结果 HbAlc 和非 HbAlc 成功分离，峰型清晰锐利且能对 HbAlc 进行定量分析。结论毛细管电泳法具有特异性好、精密度和准确度高等优点，因此，该法应该在临床上被推广应用。
목적：탐토모세관전영재분석당기화혈홍단백수평방면적림상응용개치。방법해문이용장30 cm、내경25μm 적미도층용융석영모세관，이용혈홍단백재415 nm 파장처적특이성흡수봉，이겁관진렬（PDA）법검측건강인여당뇨병급고위인군중적당기화혈홍단백（HbA1c）수평。재연구과정중，대정밀도화준학도진행료정학분석。결과 HbAlc 화비 HbAlc 성공분리，봉형청석예리차능대 HbAlc 진행정량분석。결론모세관전영법구유특이성호、정밀도화준학도고등우점，인차，해법응해재림상상피추엄응용。
Objective To discuss the application value of the capillary electrophoresis in detecting the glycosylation hemoglobin (HbA1c)level.Methods By utilizing the uncoated fused silica capillary column(30 cm in length and 25 μm in diameter)and the specific adsorption peak,the HbA1c levels in the healthy people,patients with diabetes mellitus(DM)and high risk people were de-tected by the photodiode array(PDA)method at the wavelength of 415 nm.During the research process,the precision and accuracy were perfomred the accuracy analysis.Results HbA1c could be effectively separated from non-HbA1c,with a sharp peak.And the relative quantity of HbA1c could be determined.Conclusion The capillary electrophoresis is characterized by specificity,high precision,and high accuracy.Therefore,it should be widely applied in clinic.