国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2014年
14期
1914-1915,1918
,共3页
地中海贫血%筛查%红细胞平均体积%红细胞平均血红蛋白
地中海貧血%篩查%紅細胞平均體積%紅細胞平均血紅蛋白
지중해빈혈%사사%홍세포평균체적%홍세포평균혈홍단백
thalassemia%screening%mean corpuscular volume%mean corpuscular hemoglobin
目的：对红细胞指标红细胞平均体积（MCV）和红细胞平均血红蛋白（MCH）筛查地贫的可靠性进行比较。方法将一份 MCV 处于临界值的新鲜静脉血于1 d 内送到珠海市21家医疗机构进行全血细胞分析，然后统计分析得出室间和室内MCV 和 MCH 的变异系数（CV）和偏倚值。此外，另抽取 EDTA 抗凝静脉血10份，一分为二，观察温度和放置时间对此两个指标的影响。结果该市室间 MCV 的 CV 值（4．1％）明显大于 MCH 的 CV 值（2．8％），MCH 检测结果的合格率（100％）明显高于MCV（66．7％）（P ＜0．05）。全血标本在两种温度条件（室温与冷藏）下保存72 h，MCH 指标变化不大（P ＞0．05）。血液标本冷藏72 h 时，MCV 与即测值比较差异无统计学意义（P ＞0．05），室温保存48 h时，MCV 显著升高（P ＜0．05）。在室温和冷藏保存48 h时，两者的 MCV 差异有统计学意义（P ＜0．05）。结论在室间以及在不同温度保存的条件下，MCH 指标较 MCV 重复性好且更稳定，MCH 指标作为临床一线地贫筛查指标比 MCV 指标更可靠。
目的：對紅細胞指標紅細胞平均體積（MCV）和紅細胞平均血紅蛋白（MCH）篩查地貧的可靠性進行比較。方法將一份 MCV 處于臨界值的新鮮靜脈血于1 d 內送到珠海市21傢醫療機構進行全血細胞分析，然後統計分析得齣室間和室內MCV 和 MCH 的變異繫數（CV）和偏倚值。此外，另抽取 EDTA 抗凝靜脈血10份，一分為二，觀察溫度和放置時間對此兩箇指標的影響。結果該市室間 MCV 的 CV 值（4．1％）明顯大于 MCH 的 CV 值（2．8％），MCH 檢測結果的閤格率（100％）明顯高于MCV（66．7％）（P ＜0．05）。全血標本在兩種溫度條件（室溫與冷藏）下保存72 h，MCH 指標變化不大（P ＞0．05）。血液標本冷藏72 h 時，MCV 與即測值比較差異無統計學意義（P ＞0．05），室溫保存48 h時，MCV 顯著升高（P ＜0．05）。在室溫和冷藏保存48 h時，兩者的 MCV 差異有統計學意義（P ＜0．05）。結論在室間以及在不同溫度保存的條件下，MCH 指標較 MCV 重複性好且更穩定，MCH 指標作為臨床一線地貧篩查指標比 MCV 指標更可靠。
목적：대홍세포지표홍세포평균체적（MCV）화홍세포평균혈홍단백（MCH）사사지빈적가고성진행비교。방법장일빈 MCV 처우림계치적신선정맥혈우1 d 내송도주해시21가의료궤구진행전혈세포분석，연후통계분석득출실간화실내MCV 화 MCH 적변이계수（CV）화편의치。차외，령추취 EDTA 항응정맥혈10빈，일분위이，관찰온도화방치시간대차량개지표적영향。결과해시실간 MCV 적 CV 치（4．1％）명현대우 MCH 적 CV 치（2．8％），MCH 검측결과적합격솔（100％）명현고우MCV（66．7％）（P ＜0．05）。전혈표본재량충온도조건（실온여랭장）하보존72 h，MCH 지표변화불대（P ＞0．05）。혈액표본랭장72 h 시，MCV 여즉측치비교차이무통계학의의（P ＞0．05），실온보존48 h시，MCV 현저승고（P ＜0．05）。재실온화랭장보존48 h시，량자적 MCV 차이유통계학의의（P ＜0．05）。결론재실간이급재불동온도보존적조건하，MCH 지표교 MCV 중복성호차경은정，MCH 지표작위림상일선지빈사사지표비 MCV 지표경가고。
Objective To compare the reliability of erythrocyte parameter mean corpuscular volume(MCV)and mean corpuscu-lar hemoglobin(MCH)for screening thalassaemia trait.Methods A fresh venous blood sample with the cut-off value of MCV was sent to 21 hospitals of Zhuhai city for conduct the full blood cell analysis within 1 d.Then the inter-and intra-coefficient of varia-tion (CV)as well as bias values of MCV and MCH were calculated and compared.In addition,10 EDTA-anticoagulant venous blood samples were divided into two parts,the effects of stored temperature and time on MCV and MCH were observed.Results The coefficient of variation(CV)of MCV (4.1%)was significantly greater than that of MCH (2.8%)among 21 laboratories,the qualification rate of MCH detection results was 100%,which was significantly higher than 66.7% of MCV (P <0.05).When the whole blood samples were stored under 2 kinds of the temperature condition(room temperature and refrigeration)for 72 h,MCH changed little (P >0.05).When these samples were stored under the refrigerated condition for 72 h,MCV had no statistically sig-nificant difference compared with the instant detection results of MCV (P >0.05),when stored at the room temperature for 48 h, MCV was significantly increased (P <0.05),MCV had statistical difference in storage for 48 h between the room temperature and the refrigeration.Conclusion Among laboratories and under different temperature conditions,the reproducibility of MCH is better than that of MCV and is more stable than MCV.MCH as the clinical first-line screening for thalassaemia is more reliable than MCV.
