CAJ | 학술논문

目的：探讨血管平滑肌细胞被感染人类巨细胞病毒后，其细胞内胆固醇脂代谢及相关基因表达的变化情况。方法通过分离原代脐动脉血管平滑肌细胞，以1个感染复数的人类巨细胞病毒攻击作为实验组，对照组则采用含3％小牛血清的DMEM/F12培养基模拟感染。检测平滑肌细胞内胆固醇含量在病毒攻击后的变化情况，以基因表达谱芯片技术检测基因表达情况。结果血管平滑肌细胞被感染人类巨细胞病毒后，细胞内相关基因表达发生显著变化，其中低密度脂蛋白受体相关蛋白10、11、12以及 HMG-CoA 合成酶，HMG-CoA 还原酶，B 型清道夫受体表达上调。载脂蛋白 A1、载脂蛋白 M 表达下调。结论人类巨细胞病毒感染血管平滑肌细胞后可能通过改变脂代谢途径的相关基因表达，增强细胞内胆固醇合成，导致胆固醇代谢失衡，从而参与动脉粥样硬化的发病过程。
목적：탐토혈관평활기세포피감염인류거세포병독후，기세포내담고순지대사급상관기인표체적변화정황。방법통과분리원대제동맥혈관평활기세포，이1개감염복수적인류거세포병독공격작위실험조，대조조칙채용함3％소우혈청적DMEM/F12배양기모의감염。검측평활기세포내담고순함량재병독공격후적변화정황，이기인표체보심편기술검측기인표체정황。결과혈관평활기세포피감염인류거세포병독후，세포내상관기인표체발생현저변화，기중저밀도지단백수체상관단백10、11、12이급 HMG-CoA 합성매，HMG-CoA 환원매，B 형청도부수체표체상조。재지단백 A1、재지단백 M 표체하조。결론인류거세포병독감염혈관평활기세포후가능통과개변지대사도경적상관기인표체，증강세포내담고순합성，도치담고순대사실형，종이삼여동맥죽양경화적발병과정。
Objective To investigate the change of intracellular cholesterol lipid metabolism and related gene expression after vascular smooth muscle cells infection by human cytomegalovirus(HCMV).Methods By separating the primary umbilical artery vascular smooth muscle cells with a multiplicity of infection of HCMV attack as the experimental group and the control group a-dopted the DMEM/F12 medium containing 3% bovine serum for simulating infection.The change of cholesterol content in the smooth muscle cells after virus attack was detected and the gene expression gene was detected by the gene expression profiling chip method.Results After vascular smooth muscle cells were infected by HCMV,the intracellular gene expression changed significant-ly,in which low-density lipoprotein receptor-related protein 10,11,12 and HMG-CoA synthase,HMG-CoA reductase and B scaven-ger receptor were upregulated.Apolipoprotein A1 and apolipoprotein M were downregulated.Conclusion HCMV infecting vascular smooth muscle cells may alter the lipid metabolism pathway related gene expression,enhance intracellular cholesterol synthesis,re-sult in the cholesterol metabolism imbalance,thus participate in the pathogenesis process of atherosclerosis.