CAJ | 학술논문

目的：探索一种基于16S rRNA 基因的细菌快速鉴定方法，为临床未知病原菌的诊断及治疗提供科学依据。方法对临床患者的痰标本分离培养纯菌落，直接以菌液为模板，以通用引物 PCR 扩增未知菌的16S rRNA 基因片段，产物直接测序。将测序结果进行 BLAST 比对，根据序列同源性鉴定病原细菌。结果未知病原菌经本实验鉴定为人苍白杆菌，经 ABI 细菌快速鉴定板条检测，确认结果一致。结论该研究简化了临床标本未知病原菌分离培养鉴定的步骤，建立了一种利用16S rRNA 基因扩增快速鉴定病原菌的简便方法。
목적：탐색일충기우16S rRNA 기인적세균쾌속감정방법，위림상미지병원균적진단급치료제공과학의거。방법대림상환자적담표본분리배양순균락，직접이균액위모판，이통용인물 PCR 확증미지균적16S rRNA 기인편단，산물직접측서。장측서결과진행 BLAST 비대，근거서렬동원성감정병원세균。결과미지병원균경본실험감정위인창백간균，경 ABI 세균쾌속감정판조검측，학인결과일치。결론해연구간화료림상표본미지병원균분리배양감정적보취，건립료일충이용16S rRNA 기인확증쾌속감정병원균적간편방법。
Objective To explore a rapid bacterial identifying method based on the 16S rRNA gene sequence analysis technology to provide the scientific basis for the diagnosis and treatment of unknown pathogenic bacteria.Methods The pure colonies were iso-lated and cultured directly from a clinical patient′s sputum sample.The colony as a template for PCR amplification with universal primers to amplify 16S rRNA gene fragments of unknown bacteria.The product of PCR was sequenced directly,then the sequence result was compared by using the BLAST of NCBI and the pathogen was identified based on the sequence homology.Results 1 strain of unknown pathogen was identified as ochrobactrum by this test and confirmed by ABI bacterial rapid identification sys-tem.Conclusion This study simplifies the isolation and identification procedures of unknown pathogen from the clinical samples and establishes a simple method for the rapid identification of pathogens by using 16S rRNA gene amplification.