CAJ | 학술논문

目的：探讨银杏叶总黄酮对大鼠心肌缺血再灌注损伤的保护作用及其机制。方法：取成熟健康雄性大鼠50只，随机分为即假手术组、缺血再灌注组、银杏叶总黄酮高剂量组（200mg·kg-1）、中剂量组（100mg·kg-1）、低剂量组（50mg·kg-1）。银杏叶总黄酮组分别于术前灌胃给药，1次/d。连续7d。假手术组和模型组于同一时间灌等量的0.9%氯化钠注射液。采用在体阻断大鼠左冠状动脉30min恢复灌注180min制备心肌缺血再灌注损伤模型。测定心肌梗死面积；测定血清中超氧化物歧化酶（SOD）、谷胱甘肽过氧化酶（GSH-Px）的活性及丙二醛（MDA）、谷胱甘肽（GSH）含量。结果：与模型组比较，银杏叶总黄酮组缩小心肌梗死面积，同时使血清中丙二醛含量减少，超氧化物歧化酶和谷胱甘肽过氧化酶的活性增加及谷胱甘肽含量增加。而且保护作用随着剂量的增加更加明显。结论：银杏叶总黄酮对大鼠缺血再灌注的心肌能够减少心肌梗死面积，具有不同程度的保护作用，其机制可能与抗氧化作用有关。
목적：탐토은행협총황동대대서심기결혈재관주손상적보호작용급기궤제。방법：취성숙건강웅성대서50지，수궤분위즉가수술조、결혈재관주조、은행협총황동고제량조（200mg·kg-1）、중제량조（100mg·kg-1）、저제량조（50mg·kg-1）。은행협총황동조분별우술전관위급약，1차/d。련속7d。가수술조화모형조우동일시간관등량적0.9%록화납주사액。채용재체조단대서좌관상동맥30min회복관주180min제비심기결혈재관주손상모형。측정심기경사면적；측정혈청중초양화물기화매（SOD）、곡광감태과양화매（GSH-Px）적활성급병이철（MDA）、곡광감태（GSH）함량。결과：여모형조비교，은행협총황동조축소심기경사면적，동시사혈청중병이철함량감소，초양화물기화매화곡광감태과양화매적활성증가급곡광감태함량증가。이차보호작용수착제량적증가경가명현。결론：은행협총황동대대서결혈재관주적심기능구감소심기경사면적，구유불동정도적보호작용，기궤제가능여항양화작용유관。
Objective: To discuss protection effects of Yinxing leaf total flavonoid on treating myocardial ischemia-reperfusion injury. Methods:50 rats were randomly divided into 5 groups. The Yinxing leaf total flavonoid group was given gavage for 7 days. The blank group and the model group were given 0.9% sodium chloride injection. Myocardial infarction size, serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity and malondialdehyde (MDA), glutathione (GSH) content were compared. Results:The Yinxing leaf total flavonoid group has less myocardial infarction size and serum levels of malondialdehyde, more superoxide dismutase and glutathione peroxidase and glutathione content than the model group. Conclusion:The Yinxing leaf total flavonoid can reduce myocardial infarction size, shows protective effects, the mechanism may be related with the antioxidant.