CAJ | 학술논문

目的：探讨白细胞介素-17（IL-17）在病毒性心肌炎（VMC）小鼠心肌中的表达及黄芪甲甙干预对其表达的影响。方法60只雄性Balb/c小鼠随机分为对照组、模型组及低、高剂量干预组，每组15只，后3组小鼠经腹腔接种0．1mL柯萨奇病毒B3（CVB3），低、高剂量干预组接种后当天分别以0．01、0．09g/L黄芪甲甙0．1mL灌胃，第15天处死小鼠，计算各组小鼠死亡率及心脏质量/体质量（HW/BW），通过HE染色判断炎症细胞浸润与心肌坏死病理积分，荧光实时定量PCR、Westernblot检测IL-17mRNA、蛋白表达，ELISA测定心肌组织IL-6和肿瘤坏死因子-α（TNF-α）水平。结果高剂量干预组死亡率、炎症细胞浸润与心肌坏死病理积分明显低于模型组（P＜0．05）。模型组HW/BW、心肌IL-17mRNA及蛋白表达水平、心肌IL-6、TNF水平均明显高于对照组（P＜0．01）。与模型组比较，高剂量干预组HW/BW、心肌IL-17mRNA及蛋白表达水平、心肌IL-6、TNF水平明显降低（P＜0．05）。结论IL-17可能参与VMC发病过程，黄芪甲甙对VMC的治疗作用可能与其抑制IL-17介导的炎症反应有关。
목적：탐토백세포개소-17（IL-17）재병독성심기염（VMC）소서심기중적표체급황기갑대간예대기표체적영향。방법60지웅성Balb/c소서수궤분위대조조、모형조급저、고제량간예조，매조15지，후3조소서경복강접충0．1mL가살기병독B3（CVB3），저、고제량간예조접충후당천분별이0．01、0．09g/L황기갑대0．1mL관위，제15천처사소서，계산각조소서사망솔급심장질량/체질량（HW/BW），통과HE염색판단염증세포침윤여심기배사병리적분，형광실시정량PCR、Westernblot검측IL-17mRNA、단백표체，ELISA측정심기조직IL-6화종류배사인자-α（TNF-α）수평。결과고제량간예조사망솔、염증세포침윤여심기배사병리적분명현저우모형조（P＜0．05）。모형조HW/BW、심기IL-17mRNA급단백표체수평、심기IL-6、TNF수평균명현고우대조조（P＜0．01）。여모형조비교，고제량간예조HW/BW、심기IL-17mRNA급단백표체수평、심기IL-6、TNF수평명현강저（P＜0．05）。결론IL-17가능삼여VMC발병과정，황기갑대대VMC적치료작용가능여기억제IL-17개도적염증반응유관。
Objective To explore the expression of interleukin-17 (IL-17) in the murine myocardium with viral myocarditis (VMC) ,and the influence of astragaloside intervention on its expression .Methods 60 male 4-week-old Balb/c mice were randomly divided into four groups ,namely normal control group ,model control group ,low-dose and high-dose intervention groups ,15 mice in each group .Mice in the latter three groups were inoculated with 0 .1 mL coxsackie B3 virus intraperitoneally .Then ,mice in low-dose and high-dose intervention groups were treated with 0 .01 g/L and 0 .09 g/L astragaloside solution ,respectively .All mice were killed on 15 days .The mortality and heart weight/body weighty (HW/BW ) were calculated .Histological cross sections of heart were stained with hematoxylin-eosin and histopathologic scores of inflammatory cells infiltration and myocardial necrosis were eval-uated under optical microscope .The expression levels of myocardial IL-17 mRNA and protein were detected through real-time quan-titative PCR and Western blot .The contents of IL-6 and tumor necrosis factor-α(TNF-α) in the myocardium were examined by ELISA .Results The mortality and histopathologic scores of inflammatory cells infiltration and myocardial necrosis in high-dose in-tervention group were significantly lower than those in model controlgroup (P<0 .05) .Compared with normal control group ,the HW/BW ,the expression levels of myocardial IL-17 mRNA and protein as well as the contents of IL-6 and TNF-αin the myocardi-um were markedly increased in model control group(P<0 .01) ,whereas these parameters were significantly decreased in high-dose intervention group as compared to model group (P<0 .05) .Conclusion IL-17 may be involved in the pathogenesis of VMC .The therapeutic effect of astragaloside on VMC may be associated with inhibiting IL-17-mediated inflammatory response .