CAJ | 학술논문

目的：探讨胶原纤维、神经细胞黏附分子1（NCAM1）和Runt相关转录因子1（RUNX1）在心肌桥（MB）供血心肌的表达情况。方法用Massion′s染色检测胶原纤维在无动脉粥样硬化MB供血心肌（A组）、动脉粥样硬化MB供血心肌（B组）、冠状动脉粥样硬化心脏病心肌（C组）、非心源性即刻死亡心肌中的（作为对照组，D组）分布；免疫组化检测NCAM1在上述心肌的表达情况；Western bolt检测其转录因子RUNX1的表达水平。结果 Massion′s染色显示B、C组心肌间质有较多胶原纤维，部分心肌坏死，形成纤维瘢痕；A、D组心肌间质几乎无胶原纤维。NCAM1在B组心肌表达呈阳性，呈棕黄或棕褐色，主要在细胞质及细胞膜表达；C组瘢痕灶附近心肌细胞膜及细胞质中呈阳性；A组和D组心肌表达呈弱阳性或阴性。NCAM1在A、D组供血心肌表达与B、C组比较，差异有统计学意义（P＜0．05）。Western bolt检测RUNX1亦有相似的表达。结论 MB存在动脉粥样硬化将导致慢性心肌缺血。
목적：탐토효원섬유、신경세포점부분자1（NCAM1）화Runt상관전록인자1（RUNX1）재심기교（MB）공혈심기적표체정황。방법용Massion′s염색검측효원섬유재무동맥죽양경화MB공혈심기（A조）、동맥죽양경화MB공혈심기（B조）、관상동맥죽양경화심장병심기（C조）、비심원성즉각사망심기중적（작위대조조，D조）분포；면역조화검측NCAM1재상술심기적표체정황；Western bolt검측기전록인자RUNX1적표체수평。결과 Massion′s염색현시B、C조심기간질유교다효원섬유，부분심기배사，형성섬유반흔；A、D조심기간질궤호무효원섬유。NCAM1재B조심기표체정양성，정종황혹종갈색，주요재세포질급세포막표체；C조반흔조부근심기세포막급세포질중정양성；A조화D조심기표체정약양성혹음성。NCAM1재A、D조공혈심기표체여B、C조비교，차이유통계학의의（P＜0．05）。Western bolt검측RUNX1역유상사적표체。결론 MB존재동맥죽양경화장도치만성심기결혈。
Objective To investigate the express of collagen fibers ,NCAM11 and RUNX1 in blood-supply myocardium of myo-cardial bridge(MB) .Methods The Massion′s staining were conducted to reveal the morphology of collagen fibers in blood-supply myocardium among MB without artherosclerosis(group A) ,MB with artherosclerosis(group B) ,coronary artery disease(group C) , and non-cardiogenic instantly death(group D) .The expression level of NCAM1 and RUNX1 were detected among four groups using immunohistochemistry(IHC) and Western bolt ,respectively .Results There were a lot of collagen fibers in myocardium interstitial substance and/or heart muscle necrosis between group B and C ,but not almost collagen fibers between group A and D .The expres-sion of NCAM1 was positive in group B ,and mainly expressed on the cytoplasm and membranes of cardiac myocytes by IHC .group C was similar to results in vicinal myocardium with myocardial scar .However ,there was quite lowness or negative expression of NCAM1 in group A and D .Significant difference was found between group B as well as C and group A as well as D (P<0 .05) .The expression level of RUNX1 in blood-supply myocardium was almost like to the results of NCAM 1 .Conclusion MB with arthero-sclerosis might be lead to chronic myocardial ischemia .