CAJ | 학술논문

目的：探讨B7同源体3（B7H3）对肺炎链球菌（SP）脑膜炎引起的脑损伤作用。方法以BALB/C小鼠为研究对象，通过侧脑室注射建立SP脑膜炎动物模型，48只实验动物被分为4组：生理盐水组（CON组），B7H3蛋白组（B7H3组），SP组， SP＋B7H3组。术后18、48、72 h ，小鼠被行神经行为学评分，麻醉后颈椎脱臼处死，冰上断头取脑，制备脑组织匀浆，运用实时-PCR法，检测神经元特异性烯醇化酶（NSE）、S100b mRNA的表达。结果 B7H3组的神经行为学评分与CON组比较，差异无统计学意义（P＞0．05）；SP组明显低于CON组（P＜0．05）；SP＋B7H3组较SP组更进一步降低（P＜0．05）。B7H3组和CON组的NSE、S100b mRNA相对表达量差异无统计学意义（P＞0．05）；不同时间点SP组NSE、S100b mRNA相对表达量高于CON组（P＜0．05）；SP＋B7H3组NSE、S100b mRNA相对表达量与SP组比较，各时间点更进一步升高（P＜0．05）。结论 B7H3在SP诱导的小鼠脑膜炎中，上调NSE、S100b的基因表达，促进了脑膜炎病情的进展。
목적：탐토B7동원체3（B7H3）대폐염련구균（SP）뇌막염인기적뇌손상작용。방법이BALB/C소서위연구대상，통과측뇌실주사건립SP뇌막염동물모형，48지실험동물피분위4조：생리염수조（CON조），B7H3단백조（B7H3조），SP조， SP＋B7H3조。술후18、48、72 h ，소서피행신경행위학평분，마취후경추탈구처사，빙상단두취뇌，제비뇌조직균장，운용실시-PCR법，검측신경원특이성희순화매（NSE）、S100b mRNA적표체。결과 B7H3조적신경행위학평분여CON조비교，차이무통계학의의（P＞0．05）；SP조명현저우CON조（P＜0．05）；SP＋B7H3조교SP조경진일보강저（P＜0．05）。B7H3조화CON조적NSE、S100b mRNA상대표체량차이무통계학의의（P＞0．05）；불동시간점SP조NSE、S100b mRNA상대표체량고우CON조（P＜0．05）；SP＋B7H3조NSE、S100b mRNA상대표체량여SP조비교，각시간점경진일보승고（P＜0．05）。결론 B7H3재SP유도적소서뇌막염중，상조NSE、S100b적기인표체，촉진료뇌막염병정적진전。
Objective To investigate the effect of B7 Homology 3(B7H3)on brain damage of S .Pneumococcal(SP)meningitis . Methods SP meningitis was established by intracerebral ventricular injection of SP suspension on wild-type BALB/C mice .48 mice were divided into 4 groups and received following injections :NS(CON group) ,recombinant murine B7H3alone(B7H3 group) ,SP group ,SP+B7H3 group .At 18 ,48 ,72 h post infection ,mice were conducted neurobehavior score ,then they were anesthetized and killed by cervical vertebra dislocation ,brains were collected .The mRNA expressions of NSE and S100b were detected by real-time PCR .Results Compared with CON group ,the scores of recombinant murine B7H3 group had no significant change at 18 ,48 ,72 h after infection of SP(P>0 .05);at different time points the scores of SP group were decreased significantly than the CON group (P<0 .05);scores of SP+B7H3 group decreased furtherly than SP group(P<0 .05) .The relative expressions of NSE ,S100b mR-NA in brain tissue homogenate :for the NSE ,S100b mRNA relative expressions ,there was no significant difference between B7H3 and CON group at 18 ,48 ,72 h post SP injection(P>0 .05) .At 18h ,48h ,72h ,post infection mRNA expressions of NSE ,S100b in SP group increased compared with CON group(P<0 .05);the mRNA expressions of NSE ,S100b increased furtherly in SP+B7H3 group compared with SP group(P<0 .05) .Conclusion B7H3 upregulates the mRNA expressions of NSE and S100b ,and promotes the progress of SP meningitis in mice .