重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2014年
19期
2428-2431
,共4页
支力强%马巍%王维华%王小平
支力彊%馬巍%王維華%王小平
지력강%마외%왕유화%왕소평
肾上腺髓质素%整合素αvβ3%人骨肉瘤
腎上腺髓質素%整閤素αvβ3%人骨肉瘤
신상선수질소%정합소αvβ3%인골육류
adrenomedullin%integrinαvβ3%osteosarcoma
目的：研究肾上腺髓质素（ADM ）和整合素αvβ3在人骨肉瘤组织的不同临床分期、病理分级及组织学分型中的表达情况，并探讨二者之间的关系。方法应用免疫组化SABC法和图像分析技术的方法检测30例骨肉瘤组织中ADM 和整合素αvβ3表达的阳性率、强度和平均光密度（ALD）。每张切片分别由两名病理医师在未知临床和病理资料的情况下独立观察切片，随机观察（至少5个具有代表性的高倍视野，计数100个细胞）判定肿瘤分期、分级及细胞染色程度。结果 ADM 与整合素αvβ3表达的阳性率和表达强弱均随着骨肉瘤分期增加而上调（P＜0．05），并在各期之间的差异有统计学意义（P＜0．05）。ADM 与整合素αvβ3的表达水平呈正相关。结论 ADM和整合素αvβ3在骨肉瘤中的表达强弱与骨肉瘤的恶性程度密切相关，且二者的表达变化呈正相关。
目的：研究腎上腺髓質素（ADM ）和整閤素αvβ3在人骨肉瘤組織的不同臨床分期、病理分級及組織學分型中的錶達情況，併探討二者之間的關繫。方法應用免疫組化SABC法和圖像分析技術的方法檢測30例骨肉瘤組織中ADM 和整閤素αvβ3錶達的暘性率、彊度和平均光密度（ALD）。每張切片分彆由兩名病理醫師在未知臨床和病理資料的情況下獨立觀察切片，隨機觀察（至少5箇具有代錶性的高倍視野，計數100箇細胞）判定腫瘤分期、分級及細胞染色程度。結果 ADM 與整閤素αvβ3錶達的暘性率和錶達彊弱均隨著骨肉瘤分期增加而上調（P＜0．05），併在各期之間的差異有統計學意義（P＜0．05）。ADM 與整閤素αvβ3的錶達水平呈正相關。結論 ADM和整閤素αvβ3在骨肉瘤中的錶達彊弱與骨肉瘤的噁性程度密切相關，且二者的錶達變化呈正相關。
목적：연구신상선수질소（ADM ）화정합소αvβ3재인골육류조직적불동림상분기、병리분급급조직학분형중적표체정황，병탐토이자지간적관계。방법응용면역조화SABC법화도상분석기술적방법검측30례골육류조직중ADM 화정합소αvβ3표체적양성솔、강도화평균광밀도（ALD）。매장절편분별유량명병리의사재미지림상화병리자료적정황하독립관찰절편，수궤관찰（지소5개구유대표성적고배시야，계수100개세포）판정종류분기、분급급세포염색정도。결과 ADM 여정합소αvβ3표체적양성솔화표체강약균수착골육류분기증가이상조（P＜0．05），병재각기지간적차이유통계학의의（P＜0．05）。ADM 여정합소αvβ3적표체수평정정상관。결론 ADM화정합소αvβ3재골육류중적표체강약여골육류적악성정도밀절상관，차이자적표체변화정정상관。
Objective To study the expression and relationship between adrenomedullin (ADM )and integrinαvβ3 in different pathological grades ,clinical stages and histologic subtypes of human osteosarcoma .Methods The expression positiverate ,intensity , the average optical density (ALD) of ADM and integrinαvβ3 in 30 osteosarcomacases was detected with immunohistochemical SABC technique and image analysis system .Each pathological section observed by two pathologists respectively in the case of unknown clinical and pathological information .At least five representative high field of vision counting 100 cells observed at random ,in this way the degree of tumor grade ,stage and cell staining were determined by two pathologist who unknown clinical and pathological data .Results The positive rate and intensity of ADM and integrinαvβ3 were significantly increased with the increase of osteosarco-ma stage(P<0 .05) and the difference was significant in each stage(P<0 .05) .The expression of ADM was positive correlated with the expression of integrinαvβ3 .Conclusion The expression of ADM and integrinαvβ3 in different clinicopathological grades osteosar-coma are positive associated with malignant degree of tumor ,which suggest that both of them play an important role in malignant biological behaviors of human osteosarcoma .
