CAJ | 학술논문

目的：探讨促红细胞生成素的肝细胞癌B4受体（EphB4）、血管内皮生长因子（VEGF）在食管癌中的表达及其与血管密度的关系，并分析行胸腔镜下根治术的疗效。方法采用免疫组织化学法检测76例食管癌和癌旁组织中 EphB4、VEGF的表达，使用微血管密度（MVD）值（免疫组织化学CD34染色判断）判断组织的血管密度，比较之间的关系。根据EphB4、VEGF 表达情况分析其与患者在行胸腔镜下根治术淋巴结转移率、复发率和5年生存率的关系。结果 EphB4、VEGF在癌组织的阳性表达率分别为57．89％、61．84％均显著高于癌旁组织的0和7．89％（P＜0．05）。MVD高的患者 EphB4、VEGF阳性表达率分别为67．44％和76．19％，显著高于MVD低的患者45．45％和44．11％（P＜0．05）。伴有淋巴结转移组和伴有复发组的EphB4、VEGF表达阳性率均显著高于无淋巴结转移组和无复发组的（P＜0．05）；且大于或等于5年生存率的患者中EphB4、VEGF表达阳性率分别为45．00％和45．45％，显著低于小于5年生存率的患者80．36％和85．19％，差异有统计学意义（ P＜0．05）。结论 EphB4和VEGF在食管癌中高表达，与血管密度密切相关，与淋巴结转移、复发及患者5年生存率有关；EphB4和VEGF阳性表达者术后疗效较差。
목적：탐토촉홍세포생성소적간세포암B4수체（EphB4）、혈관내피생장인자（VEGF）재식관암중적표체급기여혈관밀도적관계，병분석행흉강경하근치술적료효。방법채용면역조직화학법검측76례식관암화암방조직중 EphB4、VEGF적표체，사용미혈관밀도（MVD）치（면역조직화학CD34염색판단）판단조직적혈관밀도，비교지간적관계。근거EphB4、VEGF 표체정황분석기여환자재행흉강경하근치술림파결전이솔、복발솔화5년생존솔적관계。결과 EphB4、VEGF재암조직적양성표체솔분별위57．89％、61．84％균현저고우암방조직적0화7．89％（P＜0．05）。MVD고적환자 EphB4、VEGF양성표체솔분별위67．44％화76．19％，현저고우MVD저적환자45．45％화44．11％（P＜0．05）。반유림파결전이조화반유복발조적EphB4、VEGF표체양성솔균현저고우무림파결전이조화무복발조적（P＜0．05）；차대우혹등우5년생존솔적환자중EphB4、VEGF표체양성솔분별위45．00％화45．45％，현저저우소우5년생존솔적환자80．36％화85．19％，차이유통계학의의（ P＜0．05）。결론 EphB4화VEGF재식관암중고표체，여혈관밀도밀절상관，여림파결전이、복발급환자5년생존솔유관；EphB4화VEGF양성표체자술후료효교차。
Objective To explore the expression of EphB4 and VEGF in esophageal cancer tissues and their relationship with microvessel density (MVD ) ,and analysis the curative effect of postoperative esophageal cancer radical under thoracoscope . Methods Theexpression of EphB4 and VEGF was detected by immunohistochemistry in tumor specimens from 76 cases of esopha-geal squamous cell carcinoma and paratumor normal specimens ,used CD34 as marker to count MVD .According to the situation of expression of EphB4 and VEGF ,we analysis their relationship with lymph node metastasis rate ,recurrence and 5-year survival rate . Results The positive expression rate of EphB4 and VEGF in cancerous tissue (57 .89% and 61 .84% ) ,were significantly higher than that in tissue adjacent to carcinoma(0 and 7 .89% )(P<0 .05) .The positive expression rate ofEphB4 and VEGF in high MVD values of patients (67 .44% and 76 .19% ) ,were significantly higher than thatin low MVD values of patients (45 .45% and 44 .11% )(P<0 .05) .The positive expression rate ofEphB4 and VEGF in the patientswith lymph node metastasis group and associ-ated with recurrence ,were significantly higher than that of group without lymph node metastasis and group without recurrence (P<0 .05) .The positive expression rate of EphB4 and VEGF in patients of greater than or equal to 5 years of survival rate(45 .00% and 45 .45% ) ,were significantly lower than in patientsof Less than 5 years of survival rate (80 .36% and 85 .19% )(P<0 .05) .Conclu-sion EphB4 and VEGF are highly expressed in esophageal cancer tissue ,which may be closely associated withmicrovessel density , and lymph node metastasis ,recurrence and 5 years survival rate ;the curative effect of positive expression rate of EphB 4 and VEGF is poor .