CAJ | 학술논문

目的：分析基因型检测对诊断乙型肝炎病毒（HBV ）感染程度的临床价值。方法选取某院2011年1月至2013年8月的HBV感染者433例，采用PCR-反向点杂交法检测感染者的HBV基因型，PCR法检测感染者DNA表达载量，ELISA方法检测HBV的E抗原。结果 HBV感染患者中B基因型患者比例（68．13％）明显高于BC混合基因型（5．77％）和C基因型（26．10％），差异有统计学意义（P＜0．05）；HBV患者DNA表达载量在不同基因型间差异无统计学意义（P＞0．05）；但HBV E抗原阴性率在不同基因型之间有所不同，B基因型23．82％，BC混合基因型14．78％，C基因型1．42％，差异有统计学意义（ P＜0．05）；基因型与病情严重程度有关：轻中度乙型肝炎患者B基因型比例最高（87．20％），其次为C基因型（9．34％）和BC混合基因型（3．46％）、重度乙型肝炎分别为C基因型（77．08％）、BC混合基因型（14．58％）、B基因型（8．33％）。结论基因型与感染的严重程度及 HBV E抗原阴性率有关，与DNA表达载量无关。
목적：분석기인형검측대진단을형간염병독（HBV ）감염정도적림상개치。방법선취모원2011년1월지2013년8월적HBV감염자433례，채용PCR-반향점잡교법검측감염자적HBV기인형，PCR법검측감염자DNA표체재량，ELISA방법검측HBV적E항원。결과 HBV감염환자중B기인형환자비례（68．13％）명현고우BC혼합기인형（5．77％）화C기인형（26．10％），차이유통계학의의（P＜0．05）；HBV환자DNA표체재량재불동기인형간차이무통계학의의（P＞0．05）；단HBV E항원음성솔재불동기인형지간유소불동，B기인형23．82％，BC혼합기인형14．78％，C기인형1．42％，차이유통계학의의（ P＜0．05）；기인형여병정엄중정도유관：경중도을형간염환자B기인형비례최고（87．20％），기차위C기인형（9．34％）화BC혼합기인형（3．46％）、중도을형간염분별위C기인형（77．08％）、BC혼합기인형（14．58％）、B기인형（8．33％）。결론기인형여감염적엄중정도급 HBV E항원음성솔유관，여DNA표체재량무관。
Objective To investigate the value of genotype detection for diagnosis of hepatitis B virus (HBV ) infection . Methods 433 HBV pattients from January 2011 to August 2013 were detected by PCR-reverse dot blot hybridization ;the DNA were assaied by PCR ;the HBeAg were tested by ELISA .Results Of the 432 HBV patients ,the rato of genotypes B (68 .13% ) was significantly higher than that of genotypes BC (5 .77% ) and of genotypes C(26 .10% )(P<0 .05);there were no significiant differ-ence in the copy of the HBV DNA among the various genotype (P>0 .05);HBeAg negative rate of genotypes B (23 .82% ) ,geno-types BC(14 .78% ) ,genotypes C(1 .42% )had statistically significant(P<0 .05);Genes associated with disease severity :the ratio of genotype B for patients with mild-to-moderate hepatitis B was 87 .20% ,the rato of genotype C was 9 .34% and genotype BC was 3 .46% ,while the ratio of genotype C was 77 .08% ,genotype BC was 14 .58% ,genotype B was 8 .33% in severe hepatitis B .Con-clusion The genotype of HBV is related to disease severity and the negative rate of HBeAg ,it is not associated with HBV DNA of HBV .