CAJ | 학술논문

目的：探讨不同缺氧损伤细胞模型下黑青稞籽皮类黄酮物质对缺氧损伤细胞的保护作用。方法采用柠檬酸-乙醇浸提法富集黑青稞籽皮类黄酮物质，并对类黄酮含量进行两种不同方法检测和定量，用三种不同的细胞模型验证其对缺氧损伤的保护作用。结果实验条件下得到黑青稞籽皮提取物30.3 mg/g，通过光谱分析及HPLC检测发现，籽皮中的类黄酮物质多为花青素类，花青素提取率为68.6 mg/g，其中氯化花翠素占提取物的57 mg/g；以芦丁标定类黄酮总量达到69.4 mg/g；实验所用剂量条件下，黑青稞籽皮类黄酮表现出了对三种缺氧损伤细胞的保护作用，并能有效清除活性氧自由基，提高过氧化物歧化酶的活性。结论黑青稞籽皮提取物发挥缺氧损伤保护作用的物质基础可能是花青素类黄酮，它对防护活性氧介导的细胞损伤具有重要意义。
목적：탐토불동결양손상세포모형하흑청과자피류황동물질대결양손상세포적보호작용。방법채용저몽산-을순침제법부집흑청과자피류황동물질，병대류황동함량진행량충불동방법검측화정량，용삼충불동적세포모형험증기대결양손상적보호작용。결과실험조건하득도흑청과자피제취물30.3 mg/g，통과광보분석급HPLC검측발현，자피중적류황동물질다위화청소류，화청소제취솔위68.6 mg/g，기중록화화취소점제취물적57 mg/g；이호정표정류황동총량체도69.4 mg/g；실험소용제량조건하，흑청과자피류황동표현출료대삼충결양손상세포적보호작용，병능유효청제활성양자유기，제고과양화물기화매적활성。결론흑청과자피제취물발휘결양손상보호작용적물질기출가능시화청소류황동，타대방호활성양개도적세포손상구유중요의의。
Objective To investigate the protective effects of black barley pericarp flavonoids against hypoxia in 3 hy-poxia-induced cell injury models. Methods The flavonoids were extractedfrom black barley pericarp using citric acid-alcohol maceration method. The flavonoid content was assayed by two methods and the protective effects against hy-poxia were verified by 3 cell models. Results 30.3 mg pericarp extracts per 1 g dry black barley were obtained. The spectroscopy and HPLC assays identified anthocyanidins were the main components offlavonoids. The extraction rate of anthocyanin was 68.6 mg/g, and delphinidin chloride represented 57 mg/g. The flavonoids were 69.4 mg/g of dry weight. The flavonoids present in acid-alcohol extract of black barley pericarp prevented cell from hypoxia injury by effectively eliminating reactive oxygenspecies (ROS) and increasing the activities of superoxide dismutase (SOD). Conclusion Theanthocyanin flavonoids extractedfrom black barley pericarp play a protective role in hypoxic injury, especially in controlling ROS-meditatedhypoxic cellular injury.